Chromosomal locations and exon-intron structures of enzyme genes in the AsA-GSH cycle and nitrogen metabolism in seedlings
The location distribution map and exon-intron structure maps of genes on chromosomes were generated using MapGene2Chromosome_v2.1 (MG2C_v2.1) (Chao et al., 2015) and Gene Structure Display Server 2.0 (GSDS2.0) online software (Hu et al., 2015).
2.8 | GO annotations, KEGG metabolic pathways, and heat maps of the enzyme genes in the AsA-GSH cycle and nitrogen metabolism in seedlings
The GO annotations of genes were obtained from the Ensembl database (Kinsella et al., 2011), KEGG metabolic pathways for the genes were obtained using DAVID online software (Huang et al., 2009), and heat maps of gene expression were constructed using HemI1.0 software (Deng et al., 2014).
2.9 | Statistical analysis
The data were statistically analyzed using Excel and SPSS 21.0, plotted using Origin8.5, and tested for significance using the Duncan’s multiple comparison method (p<0.05 is the level of significance).
3|