Regulatory effect of exogenous melatonin on the expression of enzyme
genes in the AsA-GSH cycle and nitrogen metabolism in rice seedlings
under NaCl stress
Jing
Xiang | LunXiao Huang | ChunYan Ju | Qian
Huang | JiaJia Han | Fei Jiang | ChunLin Peng
| HongYu Wei | YunChe Chen | ZhengWu
Zhao*
Chongqing Engineering Research Center of Specialty Crop Resource,
College of Life Sciences , Chongqing Normal University ,Chongqing , China
*Correspondence
ZhengWu Zhao, Chongqing Engineering Research Center of Specialty Crop
Resource, College of Life Sciences, Chongqing Normal University, PO Box
401, 331 Chongqing, China.
E-mail :zhaozhengwu513@sina.com
Telephone: 13983604875
Funding
information
the
National Natural Science Foundation of China
Grant Number: 31670326.
Abstract
In
this study, the regulatory effect of exogenous melatonin on the
expression of genes related to the AsA-GSH cycle and nitrogen metabolism
in seedlings under NaCl stress was determined; the homology between
enzyme genes and similar genes in other species was analyzed by applying
different concentrations of exogenous melatonin to rice seedlings under
NaCl stress; and the morphology and physiological indicators of
seedlings were determined. Exogenous melatonin was found to upregulate
the expression of OsGR3 , OsGPX1 , OsNR2 ,OsGS1 , and OsGOGAT1 in seedlings under NaCl stress, which
enhanced the enzyme activities of the AsA-GSH cycle and nitrogen
metabolism, ultimately reducing the content of reactive oxygen species
(ROS) and improving the photosynthetic rate and nitrogen efficiency. A
high homology was found when rice OsGR3 , OsGPX1 ,OsNR2 , OsGS1, and OsGOGAT1 were compared with
similar genes in Arabidopsis thaliana , Hordeum vulgare ,Zea mays , and Sorghum bicolor . Further, the location of
the gene on the chromosome, the similar gene structure, GO annotation,
and KEGG metabolic pathways were explained by the functions and
metabolic processes of OsGR3 , OsGPX1 , OsNR2 ,OsGS1 , and OsGOGAT1 . Overall, our findings indicate that
exogenous melatonin participates in the regulation of enzyme gene
expression during the AsA-GSH cycle and nitrogen metabolism, ultimately
affecting the activities of related enzymes and alleviating the
oxidative damage induced by ROS in seedlings.