Chromatographic conditions
To determine the validity period of each formulation according to its
chemical stability a HPLC method was developed and validated. A C18
Kromasil column (150 mm x 4.6 mm, 5 μm) was used. The mobile phase
consisted of a mixture of acetonitrile and water (75:25 v:v). The flow
rate was 1 ml/min. The sample injection volume was 10 μL, the column
temperature was 50ºC and the analysis time was 15 min. Rapamycin
detection was processed at 280 nm.