2.11 Data analysis
Statistical analyses were performed using IBM SPSS Statistics software (IBM SPSS Statistics, RRID:SCR_019096; version 21.0). All data are presented as the mean ± S.E.M. and were statistically analyzed by one-way analysis of variance (ANOVA) or two-way repeated-measures ANOVA according to the experimental protocol, followed by unpaired t tests and least significant difference (LSD) or Dunnett’s T3 post hoc tests, as appropriate. Probability values\(\ <\)0.05 were considered statistically significant. Groups subjected to statistical analyses had sample sizes of at least 5 animals per group (n=5), where n = number of independent values. The data and statistical analysis complied with the recommendations of the British Journal of Pharmacology on experimental design and analysis in pharmacology (Curtis et al., 2018).