2.6 Elevated plus maze test
The elevated plus maze test was performed on the day after the open field test. The apparatus was placed 50 cm above the floor in a quiet and 30 lux illuminated room. It consists of two open arms (40 × 10 cm; no sidewalls; anxiety zone) and two closed arms (sidewalls 10 cm in height; no anxiety zone) connected by a central platform (10 × 10 cm). Each rat was placed alone in the center of the maze with its head toward an open arm. Over the 5-minute session, the time spent in each arm and the frequency of entries into the open arms were determined by video tracking with SMART software (RRID:SCR_002852, version 3.0). The maze was cleaned with 75% ethanol after each test to remove olfactory cues from the apparatus.