loading page

The calcium sensor CBL7 is required for Serendipita indica -induced growth stimulation in Arabidopsis thaliana, controlling defense against the endophyte and K + homeostasis in the symbiosis
  • +10
  • Marta-Marina Pérez-Alonso,
  • Carmen Guerrero-Galán,
  • Adrián González Ortega-Villaizán,
  • Paloma Ortiz-García,
  • Sandra Scholz,
  • Hitoshi Sakakibara,
  • Takatoshi Kiba,
  • Jutta Ludwig-Müller,
  • Anne Krapp,
  • Ralf Oelmüller,
  • Jesús Vicente-Carbajosa,
  • Stephan Pollmann,
  • Patricio Ramos
Marta-Marina Pérez-Alonso
Centro de Biotecnologia y Genomica de Plantas
Author Profile
Carmen Guerrero-Galán
Centro de Biotecnologia y Genomica de Plantas
Author Profile
Adrián González Ortega-Villaizán
Centro de Biotecnologia y Genomica de Plantas
Author Profile
Paloma Ortiz-García
Centro de Biotecnologia y Genomica de Plantas
Author Profile
Sandra Scholz
Friedrich Schiller Universitat Jena Institut fur Allgemeine Botanik und Pflanzenphysiologie
Author Profile
Hitoshi Sakakibara
Rikagaku Kenkyujo Kankyo Shigen Kagaku Kenkyu Center
Author Profile
Takatoshi Kiba
Rikagaku Kenkyujo Kankyo Shigen Kagaku Kenkyu Center
Author Profile
Jutta Ludwig-Müller
Technische Universitat Dresden Institut fur Botanik
Author Profile
Anne Krapp
Institut Jean-Pierre Bourgin
Author Profile
Ralf Oelmüller
Friedrich Schiller Universitat Jena Institut fur Allgemeine Botanik und Pflanzenphysiologie
Author Profile
Jesús Vicente-Carbajosa
Centro de Biotecnologia y Genomica de Plantas
Author Profile
Stephan Pollmann
Centro de Biotecnologia y Genomica de Plantas
Author Profile
Patricio Ramos
Universidad de Talca
Author Profile

Abstract

Calcium (Ca 2+) is an important second messenger in plants. The activation of Ca 2+ signaling cascades is critical in the activation of adaptive processes in response to perceived environmental stimuli, including biotic stresses. The colonization of roots by the plant growth promoting endophyte Serendipita indica involves the increase of cytosolic Ca 2+ levels in Arabidopsis thaliana. In this study, we investigated transcriptional changes in Arabidopsis roots during symbiosis with S. indica. RNA-seq profiling disclosed the significant induction of CALCINEURIN B-LIKE 7 ( CBL7) during early- and later phases of the interaction. Consistent with the transcriptomics analysis, reverse genetic evidence and yeast two-hybrid studies highlighted the functional relevance of CBL7 and tested the involvement of a CBL7-CBL-INTERACTING PROTEIN KINASE 13 (CIPK13) signaling pathway in the establishment of the mutualistic relationship that promotes plant growth. The loss-of-function of CBL7 abolished the growth promoting effect of S. indica and affected the colonization of the root by the fungus. The subsequent transcriptomics analysis of cbl7 revealed the involvement of this Ca 2+ sensor in activating plant defense responses. Furthermore, we report on the contribution of CBL7 to potassium transport in Arabidopsis. Triggered by the differential expression of a small number of K + channels/transporter genes, we analyzed K + contents in wild-type and cbl7 plants and observed a significant accumulation of K + in root of cbl7 plants, while shoot tissues demonstrated K + depletion. Taken together, our work associates CBL7 with an important role in the mutual interaction between Arabidopsis and S. indica and links the CBL7 Ca 2+ receptor protein to K + transport.
03 May 2022Submitted to Plant, Cell & Environment
04 May 2022Assigned to Editor
04 May 2022Submission Checks Completed
10 May 2022Reviewer(s) Assigned