Fig. 4.Bactericidal and healing effect of GK-19 on MRSA inoculated onto mouse scalded dorsal skin. (n = 10) (a) Time course images of mice infected byMRSA . Infected dorsal skin of mice was treated with saline solution, AamAp1, clindamycin hydrochloride or GK-19 respectively for twice every day. The concentrations of drugs were 50 μM. (b) Quantitative analysis of wounds areas using image J software. (c, d) Time courses bacterial count in MRSA infected wounds after treated with different antimicrobial peptides. Skin tissues of different groups were sampled at day 2, 4, 8, 12 and 16. Tissue suspensions were diluted with sterile saline solution and 50 μL of tissue suspensions were then plated in MHA culture plates. Images were taken 18-24 hours after inoculation. The quantitative analysis of colonies was counted using Image J software or visual observation.