Fig. 5. Fungicidal and healing effect of GK-19 on Candida Albicans inoculated onto mouse scalded dorsal skin. (n = 10) (a) Time course images of mice infected by Candida Albicans . Infected dorsal skin of mice was treated with saline solution, AamAp1, fluconazole and GK-19 respectively for twice every day. The concentrations of drugs were 50 μM. (b) Quantitative analysis of wounds areas using image J software. (c, d) Time courses fungal count inCandida Albicans infected wounds after treated with different antimicrobial peptides. Skin tissues of different groups were sampled at day 2, 4, 6, 8, 10 and 12. Tissue suspensions were diluted with sterile saline solution and 20 μL of tissue suspensions were then plated in PDA culture plates. Images were taken 18-24 hours after inoculation. The quantitative analysis of colonies was counted using Image J software or visual observation.