Fig. 5. Fungicidal and healing effect of GK-19 on Candida
Albicans inoculated onto mouse scalded dorsal skin. (n = 10) (a) Time
course images of mice infected by Candida Albicans . Infected
dorsal skin of mice was treated with saline solution, AamAp1,
fluconazole and GK-19 respectively for twice every day. The
concentrations of drugs were 50 μM. (b) Quantitative analysis of wounds
areas using image J software. (c, d) Time courses fungal count inCandida Albicans infected wounds after treated with different
antimicrobial peptides. Skin tissues of different groups were sampled at
day 2, 4, 6, 8, 10 and 12. Tissue suspensions were diluted with sterile
saline solution and 20 μL of tissue suspensions were then plated in PDA
culture plates. Images were taken 18-24 hours after inoculation. The
quantitative analysis of colonies was counted using Image J software or
visual observation.