Plant Material
We used a population of recombinant inbred lines (RILs) derived from a
cross between P. hallii var. hallii (HAL2 genotype) andP. hallii var. filipes (FIL2 genotype) to evaluate the
genetic basis of plant-microbiome interactions. A single
F1 hybrid was used to generate a F2population which was bred by single seed descent to the
F7 generation (Khasanova et al. 2019). Parents have full
genome assemblies that are publicly available
(https://phytozome-next.jgi.doe.gov/; Lovell et al. , 2018). The
RIL population linkage map was constructed by shallow whole-genome
resequencing and is congruent with the order of the physical genome.
Seeds of 293 F7RILs and parents were sterilized, germinated, transplanted into
treatment pots in a greenhouse located at the University of Texas at
Austin (16-h days at 500 μE m −2 s−1, 28°C; 8-h nights at 24°C; full protocol in
Appendix S1).