Experimental Design
Each treatment contained 293 RILs and seven replicates of each parent for a total of 307 plants per treatment in the experiment (four treatments x 293 RILs + 56 parents =1,228 plants). Incubated soil for treatments and controls was transferred to 950 ml 3” x 8” Mini-Treepots (Stuewe and Sons, Tangent, OR), which were lined with sterile plastic bags perforated at the bottom to allow water drainage and facilitate easy root system removal. Pots for all treatments were randomized in a single block design and left in the greenhouse for acclimation in open air for two weeks before seedlings were transplanted. Plants were watered with UV sterilized tap water for the duration of the experiment.