Figure Legends
Fig. 1 Parental genotypes grown under natural conditions host
distinct microbiota. Panels: (a), principal coordinate graph based on
Bray-Curtis dissimilarities; (b), Shannon diversity of samples from
native parental habitats; (d), number of ASVs with differential
abundance between parental habitats broken down by phylum where bars to
the left indicate number of ASVs enriched in the Austin habitat, while
bars to the right indicate number of ASVs enriched in the Corpus
habitat. Color legends in b and d are consistent with panel a. Microbial
treatments differ significantly in community composition for plants
growing in the greenhouse. Panels: (d), principal coordinate graph based
on Bray-Curtis dissimilarities; (e), Shannon diversity of greenhouse
samples; (f), number of differentially abundant ASVs when comparing AI
vs. CI soil inoculum for both native and mock treatments where bars to
the left indicate the comparison for the mock treatments while bars to
the right indicate native treatments. The color scheme in panels e and f
are consistent with panel d.
Fig. 2 Effect of plant ecotype (E) and microbial treatment
(TRT) and their interaction (E*TRT) on plant functional traits. Traits:
(a), shoot biomass; (b), specific leaf area; (c), lateral root length;
(d), specific root length; (e), root tissue density; (f), first order
root length (data are means +S.E.).
Fig. 3 Genetic map of the Panicum hallii RIL population
with locations of significant trait QTL by microbial treatment.
Fig. 4 Tests of effect slices for significant Treatment x
Marker interactions for traits in the full model analysis of QTL x
treatment interactions using PROC mixed in SAS with QTL modeled on the
marker nearest the QTL peak. Panels: (a), root number at 8@33.1; (b),
root mass ratio at 3@74.9; (c), specific root length at 4@19.1; (d),
first order root length at 9@25.6; (e), lateral root length at 7@3.4;
(f), tiller number at 7@17.0; (g), root diameter at 7@2.7; (h), root
number at 7@0.3; (i), shoot biomass at 9@3.9; (j), root biomass at
9@3.9; (k), root length at 9@3.9.
Fig. 5 Pairwise epistatic QTL in the P. hallii RIL
population detected only under Austin Inoculated and Corpus Inoculated
treatments, with plotted points indicating two-locus genotype means ±
1SE for the two loci impacting root diameter. Root diameter QTL
interactions for treatments: (a) Mock Inoculated (MI); (b), Austin
Inoculated (AI); (c), Corpus Inoculated (CI).