Preparation of high purity apigenin from Adinandra nitida leaves by
antisolvent recrystallization method
High purity apigenin(AP) was prepared by antisolvent recrystallization
method from old Adinandra nitida leaves. The procedure was as follows:
Adinandra nitida leaves were extracted by boiling water, and the
extracting solution was hydrolyzed with sulfuric acid to obtain crude
apigenin(58.9%); the purity of crude apigenin was further improved by
antisolvent recrystallization method. A satisfactory purity(98.47%) and
yield(86.65%) was obtained under the optimum purifying condition.
Scanning electronic microscopy(SEM),differential scanning
calorimetry(DSC), Fourier transform infrared spectroscopy(FTIR) and
HPLC-MS/MS were investigated.