Figure 4. AsgeneDB for functional profiling of As metabolism in metagenomes from freshwater, hot spring, marine sediment, and soil (a) Comparison of the number of As metabolism gene families detected using KEGG, eggNOG, COG, arCOG, KOG and AsgeneDB in environmental samples. “***” indicates that the use of AsgeneDB is significantly different to the use of the other five databases (p < 0.001). (b) Abundances (RPKM) of key As metabolic gene families in environmental samples among KEGG, eggNOG, COG, arCOG, KOG, and AsgeneDB. Data are presented as mean ± SE of all samples (standard error, n = 43). “***” indicates that the use of AsgeneDB is significantly different to the use of the other five databases (p < 0.001). (c) Abundances of As metabolic gene families annotated by AsgeneDB in four different habitats.