Acclimation
Once two populations, (one axenic, the other with its microbiome, both
founded from the same common ancestor,) were obtained for each genotype,
all cultures were acclimated for an additional two weeks in a controlled
common garden setting to remove any maternal effects and ensure an equal
physiological starting point for all plants. Each population, consisting
of ~150 individuals, was grown in a stoppered 500mL
Erlenmeyer flask filled with 350mL of diluted Hoagland’s E-media
([N]=2750 µg L-1 and [P]= 423.5 µg
L-1) placed in controlled growth chambers of the
McGill phytotron (165 µmols m-2 s-1light, 200C, 70% relative humidity, with a 14/10
light-dark cycle).