4. Conclusions
A rapid, sensitive, and specific single-tube dual RT-PCR approach was
developed for accurate quantification of NB plasma and tumor tissue M/N
ratio. The M/N ratio in NB plasma and tumor tissue detected by
single-tube dual RT-PCR approach were consistent with each other.
Meanwhile, the M/N ratio in NB tumor tissue detected by single-tube dual
RT-PCR approach were consistent with FISH results. By dynamically
monitoring 9 MNA NB after surgery, we found the M/N ratio greater than
cut-off value before 1-2 months, illustrating that the plasmaMYCN amplification status can be used as a non-invasive indicator
of NB recurrence. In summary, plasma circulating cell-free MYCNgene is a non-invasive and prominent indicator for monitoring the
recurrence of NB.