2.2. Reagents, materials and instrument
All DNA used in this study was purchased from Sangon Biotech (Shanghai,
China) and all sequences are listed in Table S1. Diethyl pyrocarbonate
(DEPC) treated water, and dNTP were purchased from TaKaRa Biotechnology
Co. Ltd. (Dalian, China; DEPC, diethylpyrocarbonate). 2 × Ace Taq Master
Mix was purchased from Vazyme Biotech Co., Ltd (Nanjing, China). The
real-time fluorescence measurements were performed with a Biorad CFX
Opus 96 Real Time PCR System (Richmond, USA). An
OD-1000+ UV-Vis spectrophotometer (One Drop®
Technologies, China) was used for absolute quantification of DNA. A
Qsep1 bio-fragment analyzer (Jiangsu, China) was used to analysis the
amplification of PCR. All chemicals and solvents were of analytical
grade purity and were purchased from Aladdin (Shanghai, China).