Viremia and viral tissue distribution between the
immunized-challenge group and the challenge group
To further evaluate the difference in viremia and distribution in ten
tissues among different groups, serum samples from dpv 0, 7, 14, 21 and
28, dpi 3, 5, 7, 10, 14 and 21 and ten organ tissues were evaluated
using real-time PCR. The RNA copy numbers of the serum samples in groups
B1 and B2 reached their highest levels at 5 dpi and then gradually
declined in these two groups (Fig. 5A, Fig. 5C). The viremia levels at
every time point in groups A1 and A2 were significantly lower than those
in groups B1 and B2, respectively (Fig. 5A, Fig. 5C). Furthermore, the
viremia levels and the number of pigs with viremia in the homologous
protection group (groups A1 and B1) were significantly lower than those
in the heterologous protection group (groups A2 and B2) (Fig. 5A, Fig.
5C). The viral loads of ten tissues in groups A1 (except liver, stomach,
intestine, brain and tonsil) and A2 (except tonsil and lymph nodes) were
significantly lower than those in groups B1 and B2, respectively (Fig.
5A, Fig. 5C).