Outlier Gene Annotation
For the 24 outlier loci identified in the N. lapillus dataset,
four (16.7%) matched sequences in the NCBI GenBank nucleotide database
using our search criteria (Table S10). Of these, two matched to the
coding sequence of the N. lapillus estrogen receptor gene (Table
5). An additional seven outlier loci matched the N. lapillustranscriptome, of which, only one matched the UniprotKB Swiss-Prot
database with >50% identity, an uncharacterized protein
from the Golden apple snail (Pomacea canaliculate ; Table 5).
For the 143 outlier loci identified in the S. umbilicalisdataset, 11 (7.7%) matched sequences within the NCBI GenBank database
using our search criteria (Table S10. Of these 11 loci, four matched
sequences contained within coding regions: two within the predicted
islet cell autoantigen one like gene (ICA1L ) of the African grass
rat (Arvicanthis niloticus ), one within the estrogen receptor
gene of the dogwhelk (N. lapillus ), and one within the predicted
cilia- and flagella-associated protein 100 gene (CFAP100 ) of the
California sea hare (Aplysia californica; Table 5). We were not
able to annotate any further outlier loci using the N. lapillustranscriptome.