Outlier Gene Annotation
For the 24 outlier loci identified in the N. lapillus dataset, four (16.7%) matched sequences in the NCBI GenBank nucleotide database using our search criteria (Table S10). Of these, two matched to the coding sequence of the N. lapillus estrogen receptor gene (Table 5). An additional seven outlier loci matched the N. lapillustranscriptome, of which, only one matched the UniprotKB Swiss-Prot database with >50% identity, an uncharacterized protein from the Golden apple snail (Pomacea canaliculate ; Table 5).
For the 143 outlier loci identified in the S. umbilicalisdataset, 11 (7.7%) matched sequences within the NCBI GenBank database using our search criteria (Table S10. Of these 11 loci, four matched sequences contained within coding regions: two within the predicted islet cell autoantigen one like gene (ICA1L ) of the African grass rat (Arvicanthis niloticus ), one within the estrogen receptor gene of the dogwhelk (N. lapillus ), and one within the predicted cilia- and flagella-associated protein 100 gene (CFAP100 ) of the California sea hare (Aplysia californica; Table 5). We were not able to annotate any further outlier loci using the N. lapillustranscriptome.