Mitogenome sequencing, assembly and annotation
Illumina TruSeq libraries were prepared with an average insert size of
300 bp and sequenced on the Illumina NovaSeq 6000 platform (Beijing
Berry Bioinformatics Technology Co., Ltd, China) generating 150 bp
paired-end reads. The mitochondrial genome of typhlocybine species was
assembled
with NOVOPlasty
v2.7.0
(Dierckxsens et al., 2017), using COI sequences (MN661136, MN699874,
MG397188, KY039138, MT488436 and NC_046037) as seeds and the K-mer
value set to 39. Annotation and visualization of the mitogenomes was
accomplished with MitoZ
v1.04
(Meng et al., 2019). Mitogenome sequences with low-quality assembly
results were submitted to
MITOS
WebServer (Bernt et al., 2013), under default settings and the
invertebrate genetic code. Gene boundaries were defined using the ARWEN
v1.2 (Laslett & Canbäck, 2008), and Geneious Prime (Kearse et al.,
2012). The 28 newly assembled mitogenome sequences were deposited in
GenBank (Table S2).