Figure 2. Compound screening using depolarization-induced
Ca2+ influx and whole-cell patch-clamp in DRG neurons
identify various high-voltage-activated Ca2+ channels
inhibitors. (A) Percent change in average response of DRG sensory
neurons incubated overnight with 20 µM of the indicated compounds in
response to 90 mM KCl. N=61-629 cells; error bars indicate mean ± SEM.p values as indicated; One-Way ANOVA with the Dunnett post hoc
test. Only the significances for the compounds that inhibit
Ca2+2+ density. N=16-98 cells indicated in
parenthesis; error bars indicate mean ± SEM; p values as
indicated; Kruskal-Wallis test followed by Dunn’s post hoc test.