Figure 8. Single and repeated intrathecal injection of CBD3063
(0.3 µg/kg) reverse mechanical allodynia following spared nerve injury.(A) Timeline of the experimental paradigm indicating that pre-SNI
baseline measurements of withdrawal threshold were taken before nerve
injury. A single intrathecal injection of CBD3063 was applied 7 days
after SNI, and withdrawal thresholds were followed from 0.5h to 36 hours
post injection. p value as indicated; Multiple Mann-Whitney
tests. (B) Quantification of the area under the curve between 0.5 and 36
h after i.t. injection. p value as indicated; Mann-Whitney test.
N=6-10 rats per conditions; error bars indicate mean ± SEM. (C) Timeline
of the repeated i.t. injection of CBD3063 indicating that pre-SNI
baseline measurements of withdrawal threshold were taken before nerve
injury. After assessing the paw withdrawal threshold 7 days after SNI,
intrathecal injections of CBD3063 were applied for 14 consecutive days
and paw withdrawal thresholds were assessed at 1, 3, 5, 7 10, and 14
days after the first i.t. injection. p value as indicated;
Multiple Mann-Whitney tests. (D) Quantification of the area under the
curve between SNI and 14 days after first i.t. injection. p value
as indicated; Mann-Whitney test. N=5 rats per group; error bars indicate
mean ± SEM.