Data Analysis
Graphing and statistical analysis was performed with GraphPad Prism
(Version 9). All data sets were checked for normality using D’Agostino
& Pearson test. Details of statistical tests, significance and sample
sizes are reported in the appropriate figure legends. All data plotted
represent mean ± SEM. For western blot experiments, statistical
differences between groups were determined by unpaired t test or
Mann-Whitney test. Statistical significance of confocal imaging data was
evaluated by Mann-Whitney test. For electrophysiological recordings:
Normalized peak currents were analyzed by Kruskal-Wallis test followed
by the Dunn’s post hoc test; the significance of the I-V curves was
analyzed by multiple Mann-Whitney tests; peak current density as well asV1/2 midpoint potential and k slope factor
were compared using Mann-Whitney test. For resting membrane potential
and rheobase, the significance was analyzed by Mann-Whitney test; the
significance of the number of evoked action potentials per step was
analyzed by multiple Mann-Whitney tests. Behavioral data was analyzed by
multiple Mann-Whitney tests and Mann-Whitney test for time-course
experiments and area under the curve respectively. Detailed statistical
analyses are presented in Table S1 .