Figure 8. Single and repeated intrathecal injection of CBD3063 (0.3 µg/kg) reverse mechanical allodynia following spared nerve injury.(A) Timeline of the experimental paradigm indicating that pre-SNI baseline measurements of withdrawal threshold were taken before nerve injury. A single intrathecal injection of CBD3063 was applied 7 days after SNI, and withdrawal thresholds were followed from 0.5h to 36 hours post injection. p value as indicated; Multiple Mann-Whitney tests. (B) Quantification of the area under the curve between 0.5 and 36 h after i.t. injection. p value as indicated; Mann-Whitney test. N=6-10 rats per conditions; error bars indicate mean ± SEM. (C) Timeline of the repeated i.t. injection of CBD3063 indicating that pre-SNI baseline measurements of withdrawal threshold were taken before nerve injury. After assessing the paw withdrawal threshold 7 days after SNI, intrathecal injections of CBD3063 were applied for 14 consecutive days and paw withdrawal thresholds were assessed at 1, 3, 5, 7 10, and 14 days after the first i.t. injection. p value as indicated; Multiple Mann-Whitney tests. (D) Quantification of the area under the curve between SNI and 14 days after first i.t. injection. p value as indicated; Mann-Whitney test. N=5 rats per group; error bars indicate mean ± SEM.