Figure 3. Supply of Olr-/- rats with wild type PH rat plasma-derived exosomes deteriorated hypoxia-induced PH
(A) Schematic diagram of experimental design; (B) The representative echocardiographic images of PAT/PET; (C) The representative echocardiographic images of PAWT; (D-G) RVSP, PAT/PET, PAWT, RVWT, (n=8); (H) HE staining of lung tissues;(I) Immunofluorescence staining of SM22α and PCNA in lung tissues; (J) The expression of SM22α protein in pulmonary arteries was analyzed by Western blotting (n=4); (K) The expression of PCNA protein in pulmonary arteries was analyzed by Western blotting (n=4).
Fig. 4. Endogenous PASMCs LOX-1 exhibited negligible effects on PH rat plasma exosomes-elicited PASMCs phenotypic switching,proliferation and migration
(A) Immunofluorescence staining of α-SMA and PCNA in PASMCs;(B-C) The expression of α-SMA, SM22α and PCNA proteins in PASMCs was analyzed by Western blotting (n=3); (D) EdU staining to evaluate PASMCs proliferation; (E) Statistical summary of EdU staining (n=3); (F) Flow cytometry to evaluate PASMCs proliferation; (G) Statistical summary of flow cytometry (n=3);(H) PASMCs migration was evaluated by scratch wound healing assay; (I) Statistical summary of scratch wound healing assay (n=3).
Fig. 5. ERK1/2 signaling mediated plasma exosomes-induced PASMCs phenotypic switching (A-B) p-ERK1/2 and ERK1/2 in PASMCs was analyzed by Western blotting (n=3); (C-D) p-ERK1/2 and ERK1/2 in PASMCs was analyzed by Western blotting (n=3); (E-F)p-ERK1/2 and ERK1/2 in pulmonary arteries was analyzed by Western blotting (n=4); (G-H) The expression of p-ERK1/2 and ERK1/2 in PASMCs was analyzed by Western blotting (n=3); (I-J) The expression of α-SMA, SM22α and PCNA proteins in PASMCs was analyzed by Western blotting (n=3).