2.9. Proliferation assay
PASMCs were plated into 96-well or 6-well flat bottom culture plates and
treated as planned for 24 h. Then, PASMCs proliferation was quantified
by 5-Ethynyl-2’-deoxyuridine (EdU) incorporation assay kit (Ribobio,
China) and flow cytometry.
All protocols were performed
according to the manufacturers’ instructions as previously
described(Zhang et al., 2018b).