2.6. In vivo exosomes treatment
The 6-7-week-old WT and Olr1-/- rats were grouped as follows: WT rats exposed to hypoxia (10% O2) for 3 weeks (WT-Hypoxia), Olr1-/- rats exposed to hypoxia (10% O2) for 3 weeks (Olr1-/- -Hypoxia),Olr1-/- rats exposed to hypoxia (10% O2) for 3 weeks and treated with hypoxic WT rat plasma-derived exosomes (+Exos-WTHypo.),Olr1-/-rats exposed to hypoxia (10% O2) for 3 weeks and treated with hypoxic Olr1-/- rat plasma-derived exosomes (+Exos-KOHypo.). Exosomes derived from 5 ml plasma were resuspended in 300 μl PBS, and were administered via tail vein injection once daily for 3 days before hypoxia initiation. On day 7 and 14 over the course of hypoxia, exosomes derived from 5 ml plasma were also given to Olr1-/- rats. Control rats received an equal volume of PBS. Rats were evaluated 21 days after hypoxia initiation. Detection of various indicators and harvest of tissue were the same as the SD rats.