2.10. Scratch wound healing assay
PASMCs were seeded into 6-well flat bottom culture. Upon 80%
confluence, PASMCs were starved with low serum (0.1% FBS) for 24 h. The
scratches were created by drawing two parallel lines with a 1ml pipette
tips, and the images were taken at 0 h after scratches under a light
microscope. After 24 h treatment, images were taken again, and the rate
of cell migration was quantified.
2.11.Western blot analysis
Proteins were extracted from exosomes derived from 5 ml plasma,
pulmonary arteries or PASMCs lysates with RIPA buffer (containing 1%
PMSF). The detailed steps were shown in supplementary methods.