2.5. Immunogold electron microscopy
The exosomes pellets were fixed with 2% PFA and deposited onto formvar
carbon-coated electron-microscopy
grids for 20 min. The grids were transferred to 50 mM glycine three
times for 3 min each time, and then blocked with 5% BSA for 10 min.
Immunogold staining was conducted by incubating the sections with a
rabbit anti-LOX-1 (1:200, Abcam) antibody overnight at 4℃, followed by
incubation with anti-rabbit 5 nm IgG gold (1:50, Boster) for 1 h. The
grids were separately transferred into 1% glutaraldehyde and stained by
adding 2% uranyl acetate. After air dry, the grids were viewed under
electron microscopy.