2.9. Proliferation assay
PASMCs were plated into 96-well or 6-well flat bottom culture plates and treated as planned for 24 h. Then, PASMCs proliferation was quantified by 5-Ethynyl-2’-deoxyuridine (EdU) incorporation assay kit (Ribobio, China) and flow cytometry. All protocols were performed according to the manufacturers’ instructions as previously described(Zhang et al., 2018b).