2.7. Primary rat PASMCs culture
The rat primary PASMCs were cultured as previously described(Zhang et
al., 2018b; Ge et al., 2021). PASMCs were maintained in Dulbecco’s
modified Eagle’s medium (DMEM) supplemented with 20% fetal bovine serum
(FBS) and cultured at 37 °C under 5% CO2. The medium
was replaced with fresh medium after overnight incubation. The 3 to 5
passages of PASMCs were used for the experiments.