2.6. In vivo exosomes treatment
The 6-7-week-old WT and Olr1-/- rats were
grouped as follows: WT rats exposed to hypoxia (10% O2)
for 3 weeks (WT-Hypoxia), Olr1-/- rats exposed
to hypoxia (10% O2) for 3 weeks
(Olr1-/- -Hypoxia),Olr1-/- rats exposed to hypoxia (10%
O2) for 3 weeks and treated with hypoxic WT rat
plasma-derived exosomes (+Exos-WTHypo.),Olr1-/-rats exposed to hypoxia (10% O2) for 3 weeks and
treated with hypoxic Olr1-/- rat plasma-derived
exosomes (+Exos-KOHypo.). Exosomes derived from 5 ml
plasma were resuspended in 300 μl PBS, and were administered via tail
vein injection once daily for 3 days before hypoxia initiation. On day 7
and 14 over the course of hypoxia, exosomes derived from 5 ml plasma
were also given to Olr1-/- rats. Control rats
received an equal volume of PBS. Rats were evaluated 21 days after
hypoxia initiation. Detection of various indicators and harvest of
tissue were the same as the SD rats.