Southern blot analysis
The number of integrations of the T-DNA cassettes containingFbβCA3 cDNA in the Arabidopsis thaliana host genome was checked by southern blot analysis, using the nptII probe. The transgenic lines CAx2, CAx3 and CAx5 showed single bands confirming single integration of the transgene into the Arabidopsisgenome. The CAx6 line showed a double band, which means that here, there was a two-time integration of the transgene into the host genome (Fig. S2c ). VC showed the presence of a single copy of T-DNA cassette and, thus, there was no band of nptII in the wild type (WT).