Southern blot analysis
The number of integrations of the T-DNA cassettes containingFbβCA3 cDNA in the Arabidopsis thaliana host genome was
checked by southern blot analysis, using the nptII probe. The
transgenic lines CAx2, CAx3 and CAx5 showed single bands confirming
single integration of the transgene into the Arabidopsisgenome. The CAx6 line showed a double band, which means that
here, there was a two-time integration of the transgene into the host
genome (Fig. S2c ). VC showed the presence of a single copy of
T-DNA cassette and, thus, there was no band of nptII in the wild
type (WT).