Genomic DNA analysis of transgenic plants
Genomic DNA was isolated from different transgenic Arabidopsislines overexpressing FbβCA3 , and then the FbβCA3 was
amplified by PCR using 35S forward and FbβCA3 specific reverse
primers that yielded a fragment of ~0.8 kb ofFbβCA3 suggesting that the transgene had been integrated into to
the host genome (Fig. S2a) . In order to confirm the insertion
of the binary vector, without the gene in VC plants, kanamycin
(nptII ) gene was amplified by PCR, using nptII specific
forward and reverse primers. Our PCR results showed that a fragment of
~0.8 kb from the transformants contained nptII(Fig. S2b ). These confirmed individual transgenic lines were
then grown to harvest seeds. Seeds collected from these plants were
grown again in kanamycin plates to select T2 transgenic lines.
Transgenic seeds were then grown to get T4 generations in order to
obtain homozygous transgenic plants for further use.