Generation of transgenic and growth conditions
The full length FbβCA3 (Accession no-AY167113) was cloned in
pGEM-T Easy vector and subsequently, in modified pCAMBIA1304 (Pattanayak
and Tripathy, 2011), and then transformed in Arabidopsis using
agrobacterium-mediated floral dip method, as described by Kandoi et al.
(2016) (see Table S1 for details of the primer). Vector control
(VC) plants containing the null vector, pCAMBIA1304 (binary vector
without FbβCA3 cDNA) were also generated for our research. Seeds
of the transformed plants were screened on half-strength Murashige and
Skoog (MS) agar medium containing 50 μg/ml kanamycin; these were grown
up to T4 generation. Stratified Arabidopsis seeds were sown in
agropeat: vermiculite (1:4) mixture in pots and grown under
cool-white-fluorescent light (100 µmol photons m-2s-1), under 14 h light/10 h dark photoperiods, at
21±1ºC.