Enzyme activity of carbonic anhydrase
CA activity in VC was 276 µmol CO2 hydrated (mg Chl)-1 min-1; this activity, in different transgenic lines, ranged from 348 to 550 µmol CO2 hydrated (mg Chl)-1min-1. Thus, the CA activity of the transgenic lines was 1.3 to 2.0 fold higher than in the VC (Fig. 2g).
Based on their higher transgene expression, protein abundance and enzymatic activity, CAx3, and CAx5 transgenic lines were selected for further studies.