Generation of transgenic and growth conditions
The full length FbβCA3 (Accession no-AY167113) was cloned in pGEM-T Easy vector and subsequently, in modified pCAMBIA1304 (Pattanayak and Tripathy, 2011), and then transformed in Arabidopsis using agrobacterium-mediated floral dip method, as described by Kandoi et al. (2016) (see Table S1 for details of the primer). Vector control (VC) plants containing the null vector, pCAMBIA1304 (binary vector without FbβCA3 cDNA) were also generated for our research. Seeds of the transformed plants were screened on half-strength Murashige and Skoog (MS) agar medium containing 50 μg/ml kanamycin; these were grown up to T4 generation. Stratified Arabidopsis seeds were sown in agropeat: vermiculite (1:4) mixture in pots and grown under cool-white-fluorescent light (100 µmol photons m-2s-1), under 14 h light/10 h dark photoperiods, at 21±1ºC.