5. Genome structure of CQV:
Often, the total genome size of bunyaviruses ranges from 11 to 19
kb and around 80-120 nm in diameter (Schmaljohn, 1996).Bunyaviruses have tripartite genomes containing a large (L),
medium (M), and small (S) RNA segments. Among this, the L segment
encodesRNA-dependent RNA
polymerase(RdRp), which is necessary for mRNA synthesis and replication
of the viral RNA. The M segment encodes viral glycoproteins, which ison
the viral surface and help the virus in attaching and entering into the
host cell. The S segment encodes the nucleocapsidprotein. Both ends of
each gene segmentareenclosed with 5’- and 3’-UTRs of various lengths
(Ariza et al., 2013; Zhang et al., 2015).Most of the bunyaviruseshave a negative-sense L and M segment. The S segment of the genusPhlebovirus (Elliott and Brennan, 2014) and both M and S segment
of the genus Tospovirus (Lima et al., 2016)
are ambisense. The ambisense M
segment codes for glycoprotein (Gc and Gn) in the negative sense and a
nonstructural protein (NSm) in the positive sense. The ambisense S
segment codes for the viral nucleoprotein (N) in the negative sense and
a nonstructural protein (NSs) in the positive sense(Figure 3) (Lima et
al., 2016; Bouloy, 1991).