6. Pathobiology of CQV:
The ambisense genome of bunyaviruses including the CQV requires two rounds of transcription to be completed. Viral entry into its host cell occurs through the receptor-mediated endocytosis and the following stepstake place in the cytoplasm. Cell receptors are not definedfor severalbunyaviruses (Lozach et al., 2010; Santos et al., 2008; Jin et al., 2002), but those that contribute to the binding ofsomebunyaviruses contain integrins and other cell receptor proteins,e.g.,gC1qR/p32, which is expressed on dendritic cells, platelets, endothelial cells and lymphocytes (Albornoz et al., 2016). As the genome of single-stranded, negative-sense RNA viruses cannot be directly translated, the first step after permeation of the host cell and uncoating in the triggering of the virion RNA polymerase and its transcription of viral mRNAs from each of the three virion RNAs.Afterward primary viral mRNA transcription and translation, replication of the virion RNA takes place and the second round of transcription instigates, with favoredamplification of the genes that encode structural proteins required for virion synthesis. (https://www.sciencedirect.com/science/article/pii/B9780128009468000222).Virions mature through budding using intracytoplasmic vesicles related to the Golgi complex and are released by the transport of vesicles through the cytoplasm and consequent exocytosis from the plasma membranes (Figure-4) (Weber et al., 2002; https://en.wikipedia.org/wiki/Bunyavirales).