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\begin{document}

\title{Chemokine Receptors Expression on Peripheral CD4-Lymphocytes in
Rheumatoid Arthritis: CD4+CD183+ As A Diagnostics Marker for Disease
Activity in Rheumatoid Arthritis}



\author[1]{Samuel  Asamoah Sakyi}%
\author[1]{Tonnies  Abeku Buckman}%
\author[1]{Kwame Yeboah-Mensah}%
\author[2]{Daniel Antwi-Berko}%
\author[1]{Alfred Effah}%
\author[1]{Ebenezer Senu}%
\author[3]{Dzifa Dey}%
\author[4]{Maxwell Antwi}%
\author[1]{Joseph Yorke}%
\author[1]{Andy  Boateng}%
\author[1]{Akwasi Addei}%
\author[5]{Muniru Tanko}%
\author[6]{Richard Boateng}%
\affil[1]{Kwame Nkrumah University of Science and Technology}%
\affil[2]{VU University Medical Centre Amsterdam}%
\affil[3]{Korle Bu Teaching Hospital}%
\affil[4]{Koforidua Technical University}%
\affil[5]{University for Development Studies}%
\affil[6]{Komfo Anokye Teaching Hospital}%


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  \date{June 09, 2024}


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\selectlanguage{english}
\begin{abstract}
Background: T cell chemokines and its receptors play important roles in
the development and progression of rheumatoid arthritis (RA). Their
involvement has been reported in inflammatory autoimmune diseases.
However, their role in RA in the Ghanaian population has not been
explored. We evaluated the intracytoplasmic CD4+ T cell chemokine
receptors in rheumatoid arthritis patients in Ghana and determined their
relationship with disease activity. Methods. This case control study
included 48 newly diagnosed RA patients and 30 apparent healthy controls
from the orthopaedic units of Komfo Anokye Teaching Hospital (KATH),
Kumasi and Korle-Bu Teaching Hospital (KBTH), Accra, Ghana. A
well-structured questionnaire was used to obtain sociodemographic data.
Blood samples were also collected and processed for flow cytometric
analysis. Statistical analyses were done using SPSS version 26.0 and R
programming language. Results: This study found a significant difference
in age group (p \textless{} 0.0001), marital status (p \selectlanguage{ngerman}¬= 0.0210),
occupation (p = 0.0140), educational level (p = 0.0210) and religion (p
= 0.0100) between RA patients and healthy controls. Moreover,
haemoglobin level (p = 0.0010), waist circumference (p \textless{}
0.0001) and hip circumference (p = 0.0040) were significantly different
between RA patients and healthy controls. RA patients had significantly
lower levels of CD4+CD183+ compared to the control group (p \textless{}
0.001), and was positively correlated with DAS score (r = 0.0397, p =
0.789). In Receiver Operator Characteristics analysis, CD4+CD183+ could
significantly detect rheumatoid arthritis with a very high area under
the curve (AUC = 0.687, p = 0.018). At a cut-off of 0.082, CD4+CD183+
was the best chemokine receptor for detecting RA with a sensitivity of
90.0\%, specificity of 25.9\%, a positive predictive value of 69.2\%,
and a negative predictive value of 58.3\%. Conclusion: CD4+CD183+ could
serve as useful diagnostics and disease-monitoring marker for rheumatoid
arthritis in the Ghanaian population.%
\end{abstract}\selectlanguage{ngerman}%



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\textbf{Hosted file}

\verb`RA_Manuscript_Final.docx`  available at \url{https://authorea.com/users/468886/articles/708128-chemokine-receptors-expression-on-peripheral-cd4-lymphocytes-in-rheumatoid-arthritis-cd4-cd183-as-a-diagnostics-marker-for-disease-activity-in-rheumatoid-arthritis}

\selectlanguage{english}
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