C. glu and Dios treatment ameliorated HFD-induced glucose
metabolism disorder in KK-Ay diabetic mice.
Treatment with Dios andC.glu , glucose metabolism and insulin resistance-related
signaling proteins in liver and skeletal muscle were observed by western
blot analysis. Our data showed that a continuous HFD diet can lead to
severe insulin resistance in liver (Fig. 7A-7C) and skeletal muscle
(Fig. 7D-7F), however, treatment with Dios, C.glu and
Dios+C. glu . could significantly ameliorate insulin resistance by
activating the IRS/PI3K/AKT signaling pathway. Once AKT is activated, it
participates in glucose metabolism related pathways caused by insulin.
We found that Dios promoted glycogen synthesis in liver and skeletal
muscle by phosphorylating GSK-3β and activating GS. furthermore, the
level of phosphorylation AS160 and Glut4 were significantly up-regulated
in skeletal muscle by Dios, C.glu and Dios+C. glu .
treatment.