Ethics statement
All animal experiments in this study are carried out in accordance with the Guide for the Care and Use of Laboratory Animals, which was formulated by the National Institutes of Health and approved by the Office of Experimental Animal Management Committee of Chongqing, China.
Measurement of serumbiochemical indicators and serum insulin
The serum biochemical indicators (TC, TG, LDL-C, HDL-C) were measured using a BK-800 Fully Automatic Biochemical Analyzer (BIOBASE, China) according to the manufacturer’s protocol. The serum samples were collected for serum insulin concentrations detection using a commercial ELISA kit based on the manufacturer’s instructions.