Animals and Experimental Design
Eight-week-old male C57BL/6J mice and KK-Ay diabetic mice
(C57BL/6J background) purchased from
Beijing Huafukang Biotechnology Co.,
Ltd. The animals were maintained at standard environmental conditions
temperature 25 °C, humidity 50%,
and free access to sterile food and water.
After one week of acclimation, the
mice were randomly divided into 5 groups with 6 mice in each group.
C57BL/6J mice were fed with a standard diet and KK-Ay mice were fed
with a high fat diet (HFD) for two
weeks, and the blood glucose was measured and the mice with blood
glucose≥11 mmol/L were selected as the diabetic model.
The diabetic mice were randomly
divided into four groups: (1) Model
group, treated with the vehicle; (2) L-Dios group, treated with 20
mg/kg.d Dios; (3) H-Dios group, treated with 60 mg/kg.d Dios; (4)
Metformin (Met) group, treated with
60mg/kg.d Met. For the experiment of Corynebacterium
glutamicum( C. glu)transplantation, the diabetic mice were also randomly divided into four
groups: (1) Model group, treated with the vehicle; (2) Dios group,
treated with 60 mg/kg.d Dios; (3)C. glu group, treated with
5×109CFU/100ul/10gC. glu ; (4) Dios+C. glu group, treated with 60mg/kg.d Dios
and 5×109CFU/100ul/10g C. glu for four weeks.
During the treatment period, body weight and blood glucose levels were
monitored weekly. On the last day of the experiment, fresh mouse stool
samples were directly collected in the ultra-clean workbench and
immediately stored at -80°C for
subsequent analysis. Tissues including liver and skeletal muscle were
carefully harvested and kept in liquid nitrogen and then stored at −80°C
until analysis.