Ethics statement
All animal experiments in this study are carried out in accordance with
the Guide for the Care and Use of Laboratory Animals, which was
formulated by the National Institutes of Health and approved by the
Office of Experimental Animal Management Committee of Chongqing, China.
Measurement of serumbiochemical indicators and
serum insulin
The serum biochemical indicators (TC, TG, LDL-C, HDL-C) were measured
using a BK-800 Fully Automatic Biochemical Analyzer (BIOBASE, China)
according to the manufacturer’s protocol. The serum samples were
collected for serum insulin concentrations detection using
a commercial ELISA kit based on the
manufacturer’s instructions.