C. glu and Dios treatment ameliorated HFD-induced glucose metabolism disorder in KK-Ay diabetic mice.
Treatment with Dios andC.glu , glucose metabolism and insulin resistance-related signaling proteins in liver and skeletal muscle were observed by western blot analysis. Our data showed that a continuous HFD diet can lead to severe insulin resistance in liver (Fig. 7A-7C) and skeletal muscle (Fig. 7D-7F), however, treatment with Dios, C.glu and Dios+C. glu . could significantly ameliorate insulin resistance by activating the IRS/PI3K/AKT signaling pathway. Once AKT is activated, it participates in glucose metabolism related pathways caused by insulin. We found that Dios promoted glycogen synthesis in liver and skeletal muscle by phosphorylating GSK-3β and activating GS. furthermore, the level of phosphorylation AS160 and Glut4 were significantly up-regulated in skeletal muscle by Dios, C.glu and Dios+C. glu . treatment.