FIGURE 3 AR156 prime CYP71A13 and PAD3 expression and camalexin accumulation in the leaves of wild-type Col-0 afterP. capsici, B. cinerea, and Pst DC3000 infection.
Induction of systemic resistance of Arabidopsis ecotype Col-0 wild type (WT) to P. capsici , B. cinerea, and PstDC3000 by B. cereus AR156. Plants were pretreated with 5 × 107 CFU/mL of AR156 and 0.85% NaCl for 5 d. Subsequently, the plants were inoculated with P. capsici ,B. cinerea , and Pst DC3000. (A) The camalexin production in leaves was quantified at the indicated time points after inoculation with P. capsici . (B and C) The transcript levels ofCYP71A13 and PAD3 were analyzed in leaves pretreated with AR156 or NaCl by RT-qPCR at the indicated time points after inoculation with P. capsici . (D) The camalexin production in leaves was quantified at the indicated time points after inoculation with B. cinerea . (E and F) The transcript levels of CYP71A13 and PAD3 were analyzed by RT-qPCR at the indicated time points after inoculation with B. cinerea . (G) The camalexin production in leaves was quantified at the indicated time points after inoculation with Pst DC3000. (H and I) The transcript levels of CYP71A13 and PAD3 were analyzed by RT-qPCR at the indicated time points after inoculation withPst DC3000. Data represent mean ± SD (n = 3). Statistical analyses were performed between Mock and AR156 (*, P < 0.05; **, P < 0.01; Student’s t -test).