FIGURE 6 AR156-induced ISR resistance to P. capsici,B. cinerea, and Pst DC3000 is dependent on PEN3 and PDR12.
Induction of systemic resistance of Arabidopsis ecotype Col-0 wild type (WT), pen3-3 , pdr12-2 , and pen3-3/pdr12-2mutant to P. capsici , B. cinerea , and Pst DC3000 byB. cereus AR156. Plants were pretreated with 5 × 107 CFU/mL of AR156 and 0.85% NaCl for 5 d. Subsequently, the plants were inoculated with P. capsici ,B. cinerea , and Pst DC3000. (A) The degree of P. capsici colonization at 48 hpi was determined by RT-qPCR. Primers specific for the P. capsici actin gene and the A. thalianaUBC9 gene were used. (B) The degree of B. cinereacolonization at 48 hpi was determined by RT-qPCR. Primers specific for the B. cinerea actin gene and the A. thaliana UBC9gene were used. (C) The concentration of Pst DC3000 in the leaves was counted after 3 d. Data represent mean ± SD (n = 8). One-way ANOVA with Tukey’s post hoc test was performed (P < 0.05). Different letters indicate statistically significant differences.