FIGURE 5 AR156-induced ISR resistance to different pathogens is reliant on WRKY33.
Induction of systemic resistance of Arabidopsis ecotype Col-0 wild type (WT) and wrky33 mutant to P. capsici , B. cinerea , and Pst DC3000 by B. cereus AR156. Plants were pretreated with 5 × 107 CFU/mL of AR156 and 0.85% NaCl for 5 d. Subsequently, the plants were inoculated with P. capsici , B. cinerea , and Pst DC3000. (A) The degree ofP. capsici colonization at 48 hpi was determined by RT-qPCR. Primers specific for the P. capsici actin gene and the A. thaliana UBC9 gene were used. Data represent mean ± SD (n = 8). (B) Phenotypic effects of AR156 ISR on P. capsici inArabidopsis . (C) The degree of B. cinerea colonization at 48 hpi was determined by RT-qPCR. Primers specific for the B. cinerea actin gene and the A. thaliana UBC9 gene were used. Data represent mean ± SD (n = 8). (d) Phenotypic effects of AR156 ISR on B. cinerea in Arabidopsis . (e) The concentration ofPst DC3000 in the leaves was counted after 3 d. (f) Phenotypic effects of AR156-ISR on Pst DC3000 in Arabidopsis . One-way ANOVA with Tukey’s post hoc test was performed (P < 0.05). Different letters indicate statistically significant differences.