FIGURE 4 B. cereus AR156-triggered camalexin accumulation isdependent on WRKY33 upon pathogen infection
Induction of systemic resistance of Arabidopsis ecotype Col-0 wild type (WT) and wrky33 mutant to P. capsici , B. cinerea , and Pst DC3000 by B. cereus AR156. Plants were pretreated with 5 × 107 CFU/mL of AR156 and 0.85% NaCl for 5 d. Subsequently, the plants were inoculated with P. capsici , B. cinerea , and Pst DC3000. (A, E, and C) The transcript levels of WRKY33 were analyzed in Arabidopsis ecotype Col-0 wild-type leaves pretreated with AR156 or NaCl by RT-qPCR at the indicated time points after inoculation with P. capsici , B. cinerea , and Pst DC3000, separately. (B) The camalexin production in Arabidopsis ecotype Col-0 and wrky33 leaves was quantified at the indicated time points after inoculation with P. capsici . (C and D) The transcript levels of CYP71A13 and PAD3 were analyzed in Arabidopsis ecotype Col-0 and wrky33 leaves pretreated with AR156 or NaCl by RT-qPCR at the indicated time points after inoculation with P. capsici . (F) The camalexin production in Arabidopsis ecotype Col-0 and wrky33 leaves was quantified at the indicated time points after inoculation with B. cinerea . (G and H) The transcript levels of CYP71A13 and PAD3 were analyzed by RT-qPCR at the indicated time points after inoculation withB. cinerea . (J) The camalexin production in Arabidopsisecotype Col-0 and wrky33 leaves was quantified at the indicated time points after inoculation with Pst DC3000. (K and L) The transcript levels of CYP71A13 and PAD3 were analyzed by RT-qPCR at the indicated time points after inoculation with PstDC3000. Data represent mean ± SD (n = 3). Statistical analyses were performed between Mock and AR156 (*, P < 0.05; **, P < 0.01; Student’s t -test).