FIGURE 2 AR156-triggered ISR induced the accumulation of phytoalexin synthesis and secretion-related genes.
(A) Hierarchical clustering analyses of DEGs related to phytoalexin synthesis and secretion-related genes. Color scale denotes FPKM values. (B-H) Validation of RNA-seq data for seven selected genes by RT-qPCR.AtActin1 was used as an internal control. The expression level of these genes in Col-0 was set to 1. (I) Measurement of camalexin accumulation in Arabidopsis ecotype Col-0 wild type treated with 5 × 107 CFU/mL of AR156 and 0.85% NaCl. Data represent mean ± SD (n = 3). Statistical analyses were performed between Mock and AR156 (**, P < 0.01; **, P < 0.01; Student’s t -test).