Statistical analyses
Graphpad Prism (version 7) was used to interpret experimental data and for its meaningful representation graphically. Statistical analyses of the data were also performed. 1-way ANOVA followed by Tukey’s multiple-comparison test/Bonferroni’s multiple comparison test was used. Significance was set at p<0.05 for animal experiments and p<0.01 for human studies. Results were represented as Mean±SEM (Std error of mean) for animal studies and human studies. For each study, data from three biological replicates were used. All comparisons are Normoxia (Baseline) vs. other groups.
For LC-MS analysis, Target decoy PSM validator algorithm was used in all database searches and an FDR of 0.0098 was calculated by Proteome Discoverer.