Statistical analyses
Graphpad Prism (version 7) was used to interpret experimental data and
for its meaningful representation graphically. Statistical analyses of
the data were also performed. 1-way ANOVA followed by
Tukey’s multiple-comparison
test/Bonferroni’s multiple comparison test was used. Significance was
set at p<0.05 for animal experiments and p<0.01 for
human studies. Results were represented as Mean±SEM (Std error of mean)
for animal studies and human studies. For each study, data from three
biological replicates were used.
All comparisons are Normoxia (Baseline) vs. other groups.
For LC-MS analysis, Target decoy PSM validator algorithm was used in all
database searches and an FDR of 0.0098 was calculated by Proteome
Discoverer.