Complete genome sequencing
The total RNA was used to prepare libraries for Next Generation Sequencing (NGS). Six genomes were obtained following the sequence independent single primer amplification (SISPA) method and sequenced by Ion Torrent S5 (Thermo Fisher Scientific, Rodano, Italy) as previously described (De Sabato et al., 2018b) at Istituto Superiore di Sanità (FAST service). Two complete genomes were obtained at Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati” as previously described (Pierini et al., 2021) by Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ”G. Caporale”(Teramo, Italy). The reads obtained by the NGS run were quality checked and analysed by Galaxy Aries (https://aries.iss.it) (Knijn et al., 2020). The complete genomes were submitted to the NCBI database (https://www.ncbi.nlm.nih.gov): 132 (Acc. N° OK340737), WB03VT2016 (Acc. N° OK340738), WB01VT2016 (Acc. N° OK340739), WB110VT2017 (Acc. N° OK340740), WB171VT2017 (Acc. N° OK340741), WB119VT2017 (Acc. N° OK340742), 55863/2018 (Acc. N° OK429318), 47272/2017 (Acc. N° OK429319).