2.5 Phylogenetic analysis
For genetic analysis of PCV2, nucleotide sequences and deduced amino acids from this study were aligned with different reference strains and sequences from adjacent countries, which were retrieved from the GenBank database. The alignments were conducted using the Clustal W algorithm of BioEdit 7.2.5 (https://bioedit.software.informer.com/). For phylogenetic analysis, the ORF1 and ORF2 genes were aligned with reference strains of different PCV2 genotypes, and the phylogenetic tree was constructed with MEGA version 10.2.6 using neighbor-joining algorithm and bootstrap analysis of 1000 replications (Tamura, Stecher, Peterson, Filipski, & Kumar, 2013).