2.5 Phylogenetic analysis
For genetic analysis of PCV2, nucleotide sequences and deduced amino
acids from this study were aligned with different reference strains and
sequences from adjacent countries, which were retrieved from the GenBank
database. The alignments were conducted using the Clustal W algorithm of
BioEdit 7.2.5 (https://bioedit.software.informer.com/). For phylogenetic
analysis, the ORF1 and ORF2 genes were aligned with reference strains of
different PCV2 genotypes, and the phylogenetic tree was constructed with
MEGA version 10.2.6 using neighbor-joining algorithm and bootstrap
analysis of 1000 replications (Tamura, Stecher, Peterson, Filipski, &
Kumar, 2013).