Samples and immunoassays
Maternal serum samples were collected after the occurrence of
hypertension from the surplus aliquots of routine biochemical assay at
any point between 20+0 and 36+6 weeks. The concentrations of sFlt-1 and
PlGF were measured by an automated immunoassay platform (Cobas e411
module, Roche Diagnostic) in the central laboratory of our hospital
according to the protocol provided by the manufacturer. Intra- and
inter-coefficient variance was below 2.0% and 4.0%, respectively.
Ratio of sFlt-1 to PlGF was calculated for each sample.