4.9 Yeast one-hybrid (Y1H) assays
The full-length CDS of BES1 was cloned into the PGAD-T7 vector and the promoter of APX2, HSFA3, HSA32, HSP21 were cloned into pHIS2 vector. The constructs and empty vector controls were transformed into yeast strain Y187 and grown on SD/-Leu-Trp-His dropout plates for interaction analysis.