Figure
5. Sustained induction of several memory genes after priming is impaired
in BES1-RNAi but enhanced in bes1-D.
(A). Transcript levels of memory‐associated genes (APX2, HSA32,
HSP21, HSFA3 ) and non‐memory gene HSP101 prior priming in Col-0,bes1-D and BES1-RNAi . Values are mean ± SEM of three
biological replicates. Asterisks indicate statistically significant
differences (*P< 0.05, **P<0.01, ***P
<0.001) compared with the wild-type determined student’s t
test, ns indicates no significance.
(B-F). Transcript levels of HS memory‐associated genes (APX2,
HSA32, HSP21, HSFA3 ) and non‐memory gene HSP101 after priming in
Col-0, bes1-D and BES1-RNAi . Transcript levels determined
by qRT‐PCR were normalized to the reference gene ACTIN2 and the
respective non-HS control was harvested at the same time point. Values
are mean ± SEM of three biological replicates. Asterisks indicate
statistically significant differences (*P< 0.05,
**P<0.01, ***P <0.001) compared with the wild-type
when seedlings exposed to the same treatment time as determined by
student’s t test, ns indicates no significance.