Figure 4. Increased BES1 accumulation in bes1-D enhances thermal memory.
(A). Immuno-blot analysis of BES1 abundance using α-BES1 in col-0 andbes1-D during memory phase. Quantified relative band intensity of dephosphorylated BES1 protein was listed below using Image J. β-ACTIN in total protein extracts was used as a loading control.
(B). Phenotype of Col-0 and bes1-D under unprimed and primed conditions after recovery. Control: seedlings prior triggering; unprimed: seedlings were subjected to heat stress triggering and extended recovery; primed: seedlings were subjected to heat priming and stress triggering then extended recovery. Scale bar, 15 mm.
(C). Percentage of seedlings in different phenotype classes indicated in A. Phenotype classes were counted reference to (Sedaghatmehr et al., 2016), in short, ‘green’ represents the entire plant was almost green, ‘weak’ represents plants that were largely pale. Data shown are averages of three independent biological replicates each containing at least 100 individuals per experience group.
(D). Electrolyte leakage assay of seedlings indicated in A. Values are mean ± SEM of three biological replicates. Different letters indicate statistically significant differences (P< 0.05) compared with the control group (unprimed) exposed to the same treatment time as determined by two-way ANOVA followed by Fisher’s LSD test.