Figure 5. Sustained induction of several memory genes after priming is impaired in BES1-RNAi but enhanced in bes1-D.
(A). Transcript levels of memory‐associated genes (APX2, HSA32, HSP21, HSFA3 ) and non‐memory gene HSP101 prior priming in Col-0,bes1-D and BES1-RNAi . Values are mean ± SEM of three biological replicates. Asterisks indicate statistically significant differences (*P< 0.05, **P<0.01, ***P <0.001) compared with the wild-type determined student’s t test, ns indicates no significance.
(B-F). Transcript levels of HS memory‐associated genes (APX2, HSA32, HSP21, HSFA3 ) and non‐memory gene HSP101 after priming in Col-0, bes1-D and BES1-RNAi . Transcript levels determined by qRT‐PCR were normalized to the reference gene ACTIN2 and the respective non-HS control was harvested at the same time point. Values are mean ± SEM of three biological replicates. Asterisks indicate statistically significant differences (*P< 0.05, **P<0.01, ***P <0.001) compared with the wild-type when seedlings exposed to the same treatment time as determined by student’s t test, ns indicates no significance.