4.3 Electrolyte leakage assay
To measure electrolyte leakage caused by high temperature, seedlings
were harvested before or after heat triggering and rinsed twice with
deionized water, placed into 10-mL tubes containing 8 mL of deionized
water. Samples were placed at room temperature for 24 h and measured the
conductivity using an Electro-conductivity meter (DDSJ-308A, Lei-ci,
Shanghai, China), recorded as R1. Samples were then boiled at 100°C for
15 min and shaken at room temperature for another 1 h before the
electrical conductivity was measured again, recorded as R2. The
electrolyte leakage was calculated as: R1/R2. Three biological
replicates were performed for per simple.