4.7 Subcellular localization
The whole-genome sequence of BES1 was amplified and cloned into the pCAMBIA1302-GFP vector to generate proBES1::BES1-GFP. The vector was transformed into Agrobacterium tumefaciens strain (GV3101) and transient transformation into N. benthamiana leaves. After 48 h, the leaves were subjected to heat priming treatment, and the GFP fluorescence was detected at different time points using a fluorescence microscope (Leica, Wetzlar, Germany).