4.10 Chromatin immunoprecipitation (ChIP)-qPCR
ChIP was performed as previously described (Zhang et al., 2021). In brief, Arabidopsis seedlings harvest at 12 h after priming were collected and crosslinked with formaldehyde and used for chromatin isolation. After ultrasonic crushing, the complex of chromatins and GFP-BES1 or GFP were immunoprecipitated by anti-GFP antibody and harvested by protein A beads. Then the purified DNA was quantified by RT-qPCR using specific primer listed in supplemental table.