Figure 1. BES1 is required for BR-enhanced thermotolerance and memory response .
(A). Phenotype of Col-0 and BES1-RNAi under unprimed and primed conditions with or without BL application. Control: seedlings prior triggering; unprimed: seedlings were subjected to heat stress triggering and extended recovery; primed: seedlings were subjected to heat priming and stress triggering then extended recovery. The heat stress priming and triggering protocol was represented as schematic on the 554 right. Scale bar, 15 mm.
(B). Percentage of seedlings grown on 1/2 MS medium with or without 10 nM BL in different phenotype classes indicated in A. Phenotype classes were counted reference to(Sedaghatmehr et al., 2016), in short, ‘green’ represents the entire plant was almost green, ‘weak’ represents plants that were largely pale. Data shown are averages of three independent biological replicates each containing at least 100 individuals per experience group.
(C). Electrolyte leakage assay of Col-0 and BES1-RNAi grown on 1/2 MS with or without 10 nM BL in thermotolerance assay indicated in A. Values are mean ± SEM of three biological replicates. Different letters indicate statistically significant differences (P< 0.05) compared with the control group (unprimed) exposed to the same treatment time as determined by two-way ANOVA followed by Fisher’s LSD test.