4.7 Subcellular localization
The whole-genome sequence of BES1 was amplified and cloned into the
pCAMBIA1302-GFP vector to generate proBES1::BES1-GFP. The vector was
transformed into Agrobacterium tumefaciens strain (GV3101) and
transient transformation into N. benthamiana leaves. After 48 h,
the leaves were subjected to heat priming treatment, and the GFP
fluorescence was detected at different time points using a fluorescence
microscope (Leica, Wetzlar, Germany).