Figure 2. De novo synthesis and weakened degradation contribute to the accumulation of BES1 during the memory phase.
(A). Schematic representation of the thermomemory experimental set-up inArabidopsis .
(B). Quantitative reverse transcription–PCR revealed enhanced transcript of BES1 in the memory phase, transcripts of BES1 were normalized to ACTIN2 . Values are mean ± SEM of three biological replicates. Different letters indicate statistically significant differences (P< 0.05) compared with the control group (unprimed) exposed to the same treatment time as determined by two-way ANOVA followed by Fisher’s LSD test.
(C). BES1 promoter-GUS expression pattern in transgenicArabidopsis plants during thermomemory assays.
(D-G). Immunoblot analysis of BES1 protein in Col-0 seedlings during the thermomemory experiment. Seedlings were treated with DMSO (C), 100 μM CHX (D), 50 μM MG132 (E), 100 μM CHX+50 μM MG132 (F) after priming, samples were collected at the indicated time points. Quantified relative band intensity of BES1 protein was listed below using Image J. β-ACTIN in total protein extracts was used as a loading control.