Figure
1. BES1 is required for BR-enhanced thermotolerance and memory
response .
(A). Phenotype of Col-0 and BES1-RNAi under unprimed and primed
conditions with or without BL application. Control: seedlings prior
triggering; unprimed: seedlings were subjected to heat stress triggering
and extended recovery; primed: seedlings were subjected to heat priming
and stress triggering then extended recovery. The heat stress priming
and triggering protocol was represented as schematic on the 554 right.
Scale bar, 15 mm.
(B). Percentage of seedlings grown on 1/2 MS medium with or without 10
nM BL in different phenotype classes indicated in A. Phenotype classes
were counted reference to(Sedaghatmehr et al., 2016), in short, ‘green’
represents the entire plant was almost green, ‘weak’ represents plants
that were largely pale. Data shown are averages of three independent
biological replicates each containing at least 100 individuals per
experience group.
(C). Electrolyte leakage assay of Col-0 and BES1-RNAi grown on
1/2 MS with or without 10 nM BL in thermotolerance assay indicated in A.
Values are mean ± SEM of three biological replicates. Different letters
indicate statistically significant differences (P< 0.05)
compared with the control group (unprimed) exposed to the same treatment
time as determined by two-way ANOVA followed by Fisher’s LSD test.