Statistics
GraphPad Prism Version 9.0.1 was used for all statistical analyses of datasets.
Significance was determined using one-way ANOVA tests with multiple comparisons being made using Tukey’s multiple comparison test; a P-value <0.05 was considered significant. ROC curves and corresponding sensitivity and specificity values were generating from the ROC analysis on GraphPad Prism. As a summary measure of the ROC curves, the Youden Index (J ) was used to enable the selection of a threshold value (cut off point for positivity) for that marker, whist also determining both sensitivity and specificity of each biomarker 16, 17. Pearson R squared values and associated p values were calculated by correlation analysis on GraphPad Prism.