Statistics
GraphPad Prism Version 9.0.1 was used for all statistical analyses of
datasets.
Significance was determined using one-way ANOVA tests with multiple
comparisons being made using Tukey’s multiple comparison test; a P-value
<0.05 was considered significant. ROC curves and corresponding
sensitivity and specificity values were generating from the ROC analysis
on GraphPad Prism. As a summary measure of the ROC curves, the Youden
Index (J ) was used to enable the selection of a threshold value
(cut off point for positivity) for that marker, whist also determining
both sensitivity and specificity of each biomarker 16,
17. Pearson R squared values and associated p values were calculated by
correlation analysis on GraphPad Prism.