Peptides
The SARS-CoV-2 peptide pool consisted of 470 15mer peptides overlapping by 11-amino acids, covering the entire proteome of the nucleocapsid phosphoprotein (Miltenyi Biotec, Bergisch Gladbach, Germany), membrane glycoprotein (Miltenyi Biotec) and the spike (S1 and S2) protein (JPT Peptide Technologies, Berlin, Germany). All peptides were purified to >70% by HPLC and used at a final concentration of 0.5µg/ml/peptide.