Correlation of Immune Infiltrates in CC
We further assessed the interrelationships of immune stains by analyzing the correlation of pairwise markers. By unsupervised hierarchical clustering, Pearson correlation coefficients (R ) was showed in the two groups heatmaps which respectively revealed a dominant array of co-regulated markers, including CD3, CD8, CD56, CD68, PD1 and PD-L1 (Figure 4AC).
With pLN metastasis, the correlation were enhanced between CD3 and CD8 (R =0.51, P <0.0001), CD3 and CD68 (R =0.38,P <0.001), CD8 and CD56 (R =0.92, P <0.001), CD8 and CD68 (R =0.47, P <0.001), CD8 and PD-1 (R =0.42,P <0.001), CD68 and PD-1 (R =0.35, P <0.001) (Figure 4BD).