Patient Cohort
All procedures were ethically approved by the institutional Ethics
Review Committee of Fudan University
Shanghai Cancer center (FDCC).
Appropriate written informed consent was obtained from all patients
prior to sample collection.
A retrospective cohort study was
conducted in the Department of Gynecology Oncology, FDCC, which included
180 patients with the 2009 FIGO stage IB1-IIA2 who underwent radical
abdominal hysterectomy with or without bilateral salpingooophorectomy
and pelvic ± para-aortic lymphadenectomy from 2009 to 2012. All the
enrolled patients had undergone standard pelvic lymphadenectomy by
experienced gynecological oncologist. All the microscopic slides were
reviewed by the same professional gynecologic pathologist and were
reconfirmed by another experienced gynecologic pathologist. A total of
patients (90 with positive pLN and 90 with negative pLN) were assayed
and their tissue specimens and clinical records were retrospectively
studied.
Making Tissue
Microarray (TMA)
The 180 CC tissues (in situ) with pLN positive metastasis or negative
metastasis were prepared into TMA as previously described [27,28].