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\begin{document}

\title{Host traits, ownership behavior and risk factors of SARS-CoV-2 infection
in domestic pets in Mexico}



\author[1]{Fabiola Carolina Espinosa-Gómez}%
\author[1]{Elizabeth Bautista}%
\author[1]{Oscar Palacios-Cruz}%
\author[1]{Alejandra Téllez-Ramírez}%
\author[1]{Daniela Belem Vázquez-Briones}%
\author[1]{César Flores de los Ángeles}%
\author[1]{Héctor Aguirre-Alarcón}%
\author[1]{Carlos Antonio Abella-Medrano}%
\author[1]{Jorge Luis Escobedo-Straffón}%
\author[2]{Mario Solís-Hernández}%
\author[2]{Roberto Navarro-López}%
\author[3]{Alonso Aguirre}%
\affil[1]{Universidad Popular Autonoma del Estado de Puebla}%
\affil[2]{Servicio Nacional de Sanidad Inocuidad y Calidad Agroalimentaria}%
\affil[3]{George Mason University College of Science}%


\vspace{-1em}



  
  \date{June 03, 2024}


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\begin{abstract}
SARS-CoV-2 can infect pets under natural conditions, which raises
questions about the risk factors related with susceptibility of these
animals to infection. The status of pet infection by SARS-CoV-2 in
Mexico it is not well understood. We aimed to estimate the frequency of
positive household cats and dogs to viral RNA and antibodies for
SARS-CoV-2 during the second wave of human infections in Mexico, and to
recognize the major risk factors related to host and pet ownership
behavior. We evaluated two study groups, cats and dogs from COVID-19
infected/suspected households (n=44) and those admitted for veterinary
care for any reason at several veterinary hospitals in Puebla City,
M\selectlanguage{ngerman}éxico (n=91). Using RT-PCR, we identified the presence of SARS-CoV-2
RNA in swabs of four dogs (18.2\%) and zero cats in COVID-19
infected/suspected households, within this group 31.8\% of dogs and
27.3\% of cats tested IgG ELISA positive, and neutralizing antibodies
were detected in one dog (4.5\%) and two cats (9.1\%). In the random
group, 25.0\% of dogs and 43.6\% of cats were ELISA positive, only one
cat shown neutralizing antibodies (2.6\%). We identified no significant
hematological and biochemical abnormalities as risk factors, but we
found dynamic changes in leukocytes, lymphocytes, albumin/globulin rate,
and creatinine. Pet age \textgreater{}4 years old, other pets at home,
and daily cleaning of pet dish, were each associated with an increase of
SARS- CoV-2 infection (p\textless{}0.05). Allowing face lick, sharing
bed/food with pets, and owner tested positive or suspected of COVID-19
were not significant risk factors, but time (\textgreater{}4 hours) the
owner spent out from home during the lockdown for COVID-19 (OR= 0.37, p=
0.009), and outdoor pet food tray (OR= 0.32, p= 0.011) were negative
risks factors of SARS-CoV-2 infection in pets, suggesting that time the
owner spends with their pet is an important risk factor.%
\end{abstract}\selectlanguage{ngerman}%



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\textbf{Hosted file}

\verb`Espinosa Gomez et al_Risk factors of SARS-CoV-2 in pets Mexico_TED.docx`  available at \url{https://authorea.com/users/460462/articles/556468-host-traits-ownership-behavior-and-risk-factors-of-sars-cov-2-infection-in-domestic-pets-in-mexico}

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