Table 1 .1H-NMR isotopologue analysis results for acetate
released following saponification of isolated leaf cell wall samples
from (a ) 3 detached branches (one per tree, N = 3) treated with
10 mM 13C2-acetate solution for 2 days
as well as (b ) canopy leaf (N = 3) samples from a 2-year old
tree following continuous diurnal injections of the 10 mM13C2-solution into the xylem at the
base of the tree for 7 days (night: 70 µL/min, day: 150 µL/min).
Following saponification of the cell wall isolates, the values were
obtained by integrating the area of the free acetate signals
(corresponding to each of the four isotopologues shown in Fig.
10 ), and calculating the fraction of each acetate isotopologue to the
total (Fexperiment = peak area acetate isotopologue/peak
area of total acetate isotopologues), and reporting the ratio of
Fexp to that from natural abundance fractions
(Fnatural abundance). Note, statistically significant
changes in Fexperiment/Fnatural
abundance (*), no statistically significant changes (ns).