Genotyping assay for F2 and F3generations
QPCR and dPCR assays were used to identify RR, RS and SS genotypes of the F2 and F3 populations, a total of 168 F2 and 123 F3 populations were submitted to genotype analysis. In this research, the identification and screening of 168 F2 individuals showed that the F2 population had 45 RR, 86 RS and 37 SS plants. Among the 123 F3 population, 38 plants were genotyped as RR, 63 RS, and the remaining 22 SS and F3 populations were shown to segregate at a ratio of 1:2.86:1.73. the genotype test results are shown in Table S1 and Table S2.