Flow Cytometry for T cell response
For T cell assays, 2 x 106 PBMCs were seeded in RPMI 1640 media
supplemented with 5% AB serum for each of the 96 well plates. T cells
were activated by using a SARS-CoV-2 Spike protein-specific peptivator
and were incubated for 20 hours at 37°C 5% CO2 incubator. T cells were
stained with a viability dye and T cell surface markers antibodies
(CD3-FITC, CD4-PerCP-Cy5.5, CD8-Brilliant Violet 510, CD38- PE-Cy7,
CD69-APC, Brilliant Violet 605, CD14-APC-Cy7, CD19-APC-Cy7) (Biolegend).
PMA/Ionomycin, CMV, and DMSO were used as controls. Samples were run by
Attune Flow cytometer.