Cell culture
THP-1 human acute monocytic leukemia cells and HEK 293 cells were grown
in culture in Roswell Park Memorial Institute medium (RPMI 1640,
Invitrogen) or DMEM (Dulbecco’s Modified Eagle Medium) respectively,
supplemented with 10% fetal bovine serum (Sigma, Cat# F7524) and 1%
penicillin/streptomycin (P/S).