Calcium influx assay
Intracellular calcium measurements in cell populations were monitored by
using Fluo-8 calcium flux assay according to the manufactures protocol
(Abcam, Denmark) and fluorescence was analyzed at at Ex/Em 490/525 nm.
Depletion of intracellular stores was achieved by addition of 250nM
Thapsigargin (Tg; Sigma).