DSS-induced acute intestinal inflammation
For acute DSS-induced colitis, the mice were administrated 2.5% DSS (molecular weight, 36-50kD; MP Biomedicals) in their drinking water for 7 days, followed by ordinary tap water. Three treatment groups were included in the study: animals treated with 12,5µg peptide (250µl) IP every other day (0.5mg/kg); animals treated with saline (250µl) as control; or animals treated with 62,5µg blocker (250µl) IP every other day (2.5mg/kg). All buffers contain 1.25% DMSO. During this time period, the mice were weighted daily and subsequently sacrificed at day 10. At the time of sacrifice, colon specimens were collected for histology and qPCR analyses.