The inoculum bioreactor (from here onwards denoted as IF) of the two
pilot fermentations (from here onwards denoted as PF1 and PF2) was run
as a batch process and contained 18 kg of batch medium of which the
composition is given in Supplementary Table S6. The media were adjusted
to pH 5.0 with NH3 before inoculation. The inoculum
bioreactor was inoculated with 5 shake flask cultures of 400 g each and
then operated according to Supplementary Table S7. 8 Kg of broth from
the IF was used to inoculate each of the two PF. The PF contained 82 kg
of batch medium that was identical to the medium of the laboratory
bioreactors. The process started with a batch phase until glucose
depletion. The other operating conditions are given in Supplementary
Table S8.
The solutions for pH correction and foam remediation were 25 wt%
NH3, 98 wt% H2SO4 and
Basildon 86-013. The carbon feed solutions of the two pilot scale
fermentations are given in Table 1.