DPPH Free Radical Assay
The anti-DPPH● activity of antioxidant compounds was
examined according to the method developed by
Delfanian et al. (2015). Briefly, 100 µL
of antioxidant solutions (10–50 µM) was added to 3 mL of methanolic
solution of DPPH● (). The absorbance of solution
(Asample) was read at 517 nm against a blank
(Acontrol) after 30 min incubation at The ability of
phenolic antioxidants in radical scavenging (%) was investigated from
Eq. (1):
\(\%RSA=\frac{A_{\text{control}}-\ A_{\text{sample}}}{A_{\text{control}}}\ \times 100\)(1)
The IC50, [AH] required to scavenge 50% of
DPPH●, was determined by interpolating the linear
regression analysis.