Soybean Oil Purification
Purification of soybean oil was performed according to the
chromatographic method developed by
Yoshida et al. (1992) with a
chromatographic glass column (36 × 3.4 cm I.D.) packed with aluminum
oxide 60 (activated for 3 h at 200 °C) with a ratio of 1(oil) to 1
(absorbent). As a final step, the amount of tocopherols
(Wong et al., 1988), total phenols
(Capannesi et al., 2000), and
hydroperoxides were determined to ensure the purification process
efficiency. The purified soybean oil triacylglycerols were contained
inconsiderable values of PV (< 1 meq/kg), phenols and
tocopherols (< 1 mg/kg).