Table 1. Minibarcodes tested for amplifying aquaculture feeds and reference samples.
Locus Product size Barcoding gene Annealing temperature Reference Target taxa
AquaF2/C_FishR1 185 COI 51 Valdez-Moreno et al. 2019 fishes
AquaF3/C_FishR1 185 COI 50 Valdez-Moreno et al. 2019 fishes
FishF2/Shark COI-MINIR 127 COI 52 Fields et al. 2015, Boussarie et al. 2018 elasmobranchs
nsCOIFo 124 COI 43 Günther et al. 2018 fishes
Fish_miniA 129 COI 46 Shokralla et al. 2015 fishes
Crust2* 106 COI 55 Fernandes et al. 2017 crustaceans
MiniBar 130 COI 46 Meusnier et al. 2008 metazoa
Plank_COI 127 COI 52 Berry et al. 2017 plankton
Shark474F/FishR1+FishR2 200 COI 52 Cardenosa et al. 2017 elasmobranchs
16S_Fish* 220 16S 67 McInnes et al. 2017 fishes
Crust_16S 170 16S 51 Berry et al. 2017 crustaceans
Ceph_16S 200 16S 55 Berry et al. 2017 cephalopods
L2513/H2714 244 16S 57 Kitano et al. 2007 vertebrates
CEP 206 16S 58 Giusti et al. 2017 fishes
16Spc 295 16S 53 Giusti et al. 2017 fishes
16Svar 209 16S 58 Giusti et al. 2017 fishes
16S-H1 120 16S 61 Horreo et al. 2013 fishes
MiFish 185 12S 58 Miya et al. 2015 fishes
Teleo* 102 12S 55 Valentini et al 2016 fishes
18S_SSU3 170 18S 67 McInnes et al. 2017 metazoa
18Sn4 180 18S 55 Machida & Knowlton 2012 metazoa
Short28S 203 28S 59 Vestheim and Jarman 2008 zooplankton