Figure 1. Accumulation curve for reference species identified by metabarcoding markers. Markers are ordered with the locus that identifies the most reference taxa in the leftmost position followed iteratively by primers that recover the most additional unique taxa. Moving from left to right, each point indicates either the proportion (panel A) or count (panel B) of known species in the full reference DNA pool identified to the indicated taxonomic rank (color-coded) by the marker indicated and all preceding primers. The proportions in panel A reflect the number of taxa identified out of the total identified by all markers for a particular taxonomic rank. For species assignments, the total number identified was 61, therefore, this number represents 100% of taxa identified to species-level. All 103 taxa are identified to family. The targeted barcoding gene for each marker is noted in parentheses on the x-axis.