Statistical analysis
The whole collection of NGS data-set were showing in Table1.a.While the
control1 and selective1 were referring to data generated from SE1
control group and SE1 experiment group; the control2 and selective2 were
referring to data generated from SE2 control group and SE2 experiment
group.The reads were the raw sequencing data and the counts were the
filtered data.
For a specific crRNA sequence, its
abundance
(crRNA frequency) in a certain group could be calculated by its divided
by the summation of all crRNA counts.(Table1.b)Further, the fold-change
of a specific crRNA sequence across a screening experiment (SE1 or SE2),
representing the activity of a crRNA, could be calculated the ratio
between the frequency in the post selection sample (selective) and the
no-selection sample (control). (Table1.b)