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Transcriptomic analysis of Streptococcus agalactiae periprosthetic joint infection
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  • Hye-Kyung Cho,
  • Thao Masters,
  • Kerryl Greenwood-Quaintance,
  • Stephen Johnson,
  • Patricio Jeraldo,
  • Nicholas Chia,
  • Meng Pu,
  • Matthew P. Abdel,
  • Robin Patel
Hye-Kyung Cho
Mayo Clinic

Corresponding Author:[email protected]

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Thao Masters
Mayo Clinic
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Kerryl Greenwood-Quaintance
Mayo Clinic
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Stephen Johnson
Mayo Clinic
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Patricio Jeraldo
Mayo Clinic
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Nicholas Chia
Mayo Clinic Minnesota
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Meng Pu
Mayo Clinic
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Matthew P. Abdel
Mayo Clinic
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Robin Patel
Mayo Clinic
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Abstract

Although Streptococcus agalactiae periprosthetic joint infection (PJI) is not as prevalent as staphylococcal PJI, invasive S. agalactiae infection has recently increased in incidence. Here, RNA-Seq was used to perform transcriptomic analysis of S. agalactiae PJI using fluid derived from sonication of explanted arthroplasties of subjects with S. agalactiae PJI, with results compared to those of S. agalactiae strain NEM316 grown in vitro. 227 genes with outlier expression were found (164 up-regulated and 63 down-regulated) between PJI sonicate fluid and in vitro conditions. Functional enrichment analysis showed genes involved in mobilome and inorganic ion transport and metabolism to be most enriched. Genes involved in nickel, copper, and zinc transport, were upregulated. Among known virulence factors, cyl operon genes, encoding beta-hemolysin/cytolysin, were consistently highly expressed in PJI versus in vitro. The data presented provide insight into S. agalactiae PJI pathogenesis and may be a useful resource for the identification of novel PJI therapeutics or vaccines against invasive S. agalactiae infections.
28 Oct 2021Submitted to MicrobiologyOpen
01 Nov 2021Submission Checks Completed
01 Nov 2021Assigned to Editor
01 Nov 2021Reviewer(s) Assigned
14 Nov 2021Review(s) Completed, Editorial Evaluation Pending
16 Nov 2021Editorial Decision: Revise Minor
22 Nov 20211st Revision Received
22 Nov 2021Submission Checks Completed
22 Nov 2021Assigned to Editor
22 Nov 2021Review(s) Completed, Editorial Evaluation Pending
22 Nov 2021Editorial Decision: Accept
Nov 2021Published in MicrobiologyOpen volume 10 issue 6. 10.1002/mbo3.1256