bird types.
Based on mtDNA, the pairwise F st value between
Naumann’s thrush and the dusky thrush was 0.051, and the gene flow was
high (Nem = 4.68), while the pairwiseF st value between the two species T.
naumanni and T. ruficollis was 0.773 or 0.798, and the gene flow
was extremely low (Nem = 0.06 and 0.07,
respectively) (Table 6a). All pairwise tests between species were
significant (P < 0.05). The values ofF st and Nem between the
two species suggested that they had significant genetic differentiation.
Based on microsatellite loci, the pairwise F stvalue between Naumann’s thrush and the dusky thrush was 0.012, and the
gene flow was very high (Nem = 20.58), while the
pairwise F st value between the two speciesT. naumanni and T. ruficollis was 0.056 or 0.074, and the
gene flow was also high (Nem = 4.21 and 3.12,
respectively) (Table 6b). All pairwise tests between species were
significant (P < 0.05). The F stand Nem values between the two species suggested
that they had moderate genetic differentiation.
We performed Bayesian clustering with six microsatellite loci using
STRUCTURE software (Fig. 5). Submitting the results to the Structure
Harvester online software for the best K indicated that the population
structure was clearest when K was equal to 4 (Fig S1). However, the
STRUCTURE results showed no individual clustering in each bird taxon and
no significant differentiation, indicating that the nuclear information
of the four bird taxa was mixed.