2.1 Materials
The cancer growth-inhibiting (CGI) siRNA and other siRNAs were
synthesized by Genolution Pharmaceuticals Inc. (Table S1). (Seoul,
Korea). The Cy3-labeled siRNA was synthesized by GenePharma Co., Ltd.
(Shanghai, China). The FITC-labeled siRNA was synthesized by Bioneer Co.
(Daejeon, South Korea). R11 single peptide and RS and RT
fusion peptides in Table S2 were synthesized by GL Biochem, Ltd.
(Shanghai, China) with more than 95% purity. Dulbecco’s
phosphate-buffered saline (DPBS) and heparin sodium salt (from porcine
intestinal mucosa) were purchased from Sigma–Aldrich (MO, USA). Agarose
was purchased from Becton Dickinson and Company (Cockeysville, MD).
10,000× TopRed Nucleic Acid Gel Stain was purchased from GenomicBase
(Seoul, South Korea). Tris(hydroxymethyl) aminomethane (Tris) was
purchased from Glentham Life Sciences Ltd. (Corsham, UK). Acetic acid
(glacial) was purchased from Merck (Darmstadt, Germany). 6× DNA loading
dye was procured from Biofact Co., Ltd. (Daejeon, South Korea). For cell
cultures, Dulbecco’s modified eagle’s medium (DMEM; Corning, MA, USA),
fetal bovine serum (FBS; PAN Biotech, Bavaria, Germany), and
penicillin-streptomycin (Life Technologies, CA, USA) were procured.
Opti-MEM™ and 0.25% trypsin-EDTA (1×) were purchased from Thermo Fisher
Scientific, Inc. (MA, USA). Hoechst 33342 was purchased from Invitrogen,
Inc. (CA, USA). Phalloidin was procured from BioActs, Inc. (Incheon,
South Korea). Nuclease-free water (NFW) was purchased from Integrated
DNA Technologies, Inc (IA, USA). All cell culture flasks and plates were
purchased from NEST Biotechnology Co., Ltd (Wuxi, China). CCK cell
viability assay kit was procured from Donginbio, Inc. (Seoul, Korea).
Tri-RNA solution was purchased from Biotech Co. (Kaohsiung, Taiwan)