2.4 Stability test of NCs
The NCs were counted to confirm stability. First, 50 pmol of siRNA and
each peptide (R11, RS, RT) were mixed to form NCs in a
total volume of 120 μL in DPBS as described above. After 30 minutes of
incubation, 20 µL of each NC was loaded in a slide (Arthur Slide;
NanoEnTek, Seoul, Korea), and images were obtained using a fluorescence
cell counter (Arthur™; NanoEnTek, Seoul, Korea). The number of each
particle was calculated by the imaging program Image J (NIH,
USA).[28]