2.11 Cytocompatiblity of fusion peptides
The cytocompatibility of fusion peptides was assessed by CCK-8 cell viability assay. MDA-MB-231 and HDF-n cells were used as breast cancer and normal cells, respectively. First, 5.0 × 103MDA-MB-231 and HDF-n cells were seeded to wells of a 96-well plate and incubated for 16 hours at standard conditions. Next, 1, 2, 3, 4 μM RS peptide solutions and 10, 15, 20, 25 μM RT peptide solutions were added to each well. After 48-hour incubation at standard conditions, 10 µL of CCK solution was added to each well. After incubation for 2 hours, the absorbance at 450 nm was measured by the Epoch2 microplate spectrophotometer.