Increased Submersion Time Did Not Increase eDNA Collection
No significant differences in mean Cq values were detected over time for any of the nine trialled materials (Fig. 3; F = 1.28 for submersion time * material, df = 9, p = 0.25). Smoothing splines were fitted to the time interval data for each material, and a trend downward would be indicative that the material collected more eDNA over time. Only hemp and both sponge materials showed any decline in trendline over time.