Active eDNA Collection
We collected water for active eDNA filtration to compare to the results of passive eDNA collection. Five 1 L surface water samples were collected in sterile 1 L containers at five different times over the day and filtered with cellulose ester membranes (47 mm diameter, 0.45 µm pore size) using a peristaltic Sentino® Microbiology Pump on a clean benchtop at the aquarium facility. All water samples were taken on the first day.