2.6. Statistical analysis
For all response variables, significant differences (P< 0.05) among water regimes in each soil and significant differences among soils in each water regime were assessed by one-way analysis of variance (ANOVA). The effects of water regimes (one constant-moisture control treatment at 60% WHC and two DWC treatments of 100-20% and 80-40% WHC), soil treatments (OF, BF and BF+) and their interactions on C decomposition, fungal-to-bacterial ratio (F:B), MBC and DOC were assessed by linear mixed models (LMM). For the LMM, soil treatment and water regime were considered as fixed factors, and sample identity was regarded as a random factor. All statistical analyses were performed using IBM SPSS Statistics 20 (IBM SPSS Inc.), and graphs were drawn using SigmaPlot 14.0 (Systat Software Inc.).