DNA extraction and Sequencing
DNA was extracted from 68 inner-eggshell swabs using the NucleoSpin® 96
Soil extraction kit (Macherey-Nagel, Germany) following kit
instructions. The PCR amplification of the TEF-1α gene was performed
using Fusarium -specific primers with the cycling conditions
reported previously
(Cobo-Díaz et al.,
2019). Eight inner-eggshell swabs (four-symptomatic and
four-asymptomatic eggs) were sequenced following the Illumina MiSeq
sequencing methodology as described by Menke et al. (2017). More
detailed information is provided in supplementary material.