As detailed in Materials and Methods, a detailed characterisation of the DOME was performed to assess its capabilities. Calibrated measurements showed that 1 pixel in the camera field of view (FOV) equated in the real-world to 12 × 12 μm at 9X magnification, and 3.75 × 3.75 μm at 90X magnification .
To calculate the projection resolution, line triplets of varying thicknesses were projected and imaged (Figure \ref{993179}). The resulting intensity analysis (Figure \ref{174873}) shows that for 9X magnification, lines of 1 pixel width were observable although somewhat difficult to distinguish due to low intensity levels, with more distinct lines can be seen at separations of 2 pixels or more. The same can be seen for the 90X magnification results. Measuring across the peaks allows direct measurement of the observed projector pixel size. At both magnifications this agrees with the theoretical projector pixel size of 30 × 30 μm.