2.4 Physiological analyses of cold-treated cassava plants
Malondialdehyde (MDA) content in cassava leaves was determined by using Lipid oxidation detection kit (Beyotime) according to the manufacturer’s instructions. MDA was extracted with chilled thiobarbituric acid reagent, and then the MDA contents were calculated by subtracting the absorbance at 450 and 600 nm from that at 532 nm. Proline concentrations were measured by using Proline Assay Kit (Bioss, Beijing) according to the manufacturer’s instructions. Total protein extraction, purification, and determination were performed by Sanshu Biotechnology Co., Ltd (Jiangsu, China). Each treatment had three replications.