\documentclass[10pt]{article}

\usepackage{fullpage}
\usepackage{setspace}
\usepackage{parskip}
\usepackage{titlesec}
\usepackage[section]{placeins}
\usepackage{xcolor}
\usepackage{breakcites}
\usepackage{lineno}
\usepackage{hyphenat}





\PassOptionsToPackage{hyphens}{url}
\usepackage[colorlinks = true,
            linkcolor = blue,
            urlcolor  = blue,
            citecolor = blue,
            anchorcolor = blue]{hyperref}
\usepackage{etoolbox}
\makeatletter
\patchcmd\@combinedblfloats{\box\@outputbox}{\unvbox\@outputbox}{}{%
  \errmessage{\noexpand\@combinedblfloats could not be patched}%
}%
\makeatother


\usepackage{natbib}




\renewenvironment{abstract}
  {{\bfseries\noindent{\abstractname}\par\nobreak}\footnotesize}
  {\bigskip}

\titlespacing{\section}{0pt}{*3}{*1}
\titlespacing{\subsection}{0pt}{*2}{*0.5}
\titlespacing{\subsubsection}{0pt}{*1.5}{0pt}


\usepackage{authblk}


\usepackage{graphicx}
\usepackage[space]{grffile}
\usepackage{latexsym}
\usepackage{textcomp}
\usepackage{longtable}
\usepackage{tabulary}
\usepackage{booktabs,array,multirow}
\usepackage{amsfonts,amsmath,amssymb}
\providecommand\citet{\cite}
\providecommand\citep{\cite}
\providecommand\citealt{\cite}
% You can conditionalize code for latexml or normal latex using this.
\newif\iflatexml\latexmlfalse
\providecommand{\tightlist}{\setlength{\itemsep}{0pt}\setlength{\parskip}{0pt}}%

\AtBeginDocument{\DeclareGraphicsExtensions{.pdf,.PDF,.eps,.EPS,.png,.PNG,.tif,.TIF,.jpg,.JPG,.jpeg,.JPEG}}

\usepackage[utf8]{inputenc}
\usepackage[english]{babel}



\usepackage{float}








\begin{document}

\title{Genetic Variation in Photosynthetic Responses to Chilling Modulates
Proton Motive Force, Cyclic Electron Flow and Photosystem II
Photoinhibition}



\author[1]{Donghee Hoh}%
\author[2]{Isaac Osei-Bonsu}%
\author[3]{Abhijnan Chattopadhyay}%
\author[1]{Atsuko Kanazawa}%
\author[1]{Nicholas Fisher}%
\author[1]{Oliver Tessmer}%
\author[3]{Jeffrey Cruz}%
\author[4]{Philip Roberts}%
\author[4]{Bao Lam  Huynh }%
\author[1]{David Hall}%
\author[3]{David M. Kramer}%
\affil[1]{Michigan State University}%
\affil[2]{Crops Research Institute}%
\affil[3]{MSU-DOE Plant Research Laboratory}%
\affil[4]{University of California Riverside}%


\vspace{-1em}



  
  \date{May 25, 2024}


\begingroup
\let\center\flushleft
\let\endcenter\endflushleft
\maketitle
\endgroup





\selectlanguage{english}
\begin{abstract}
The work demonstrates the use of detailed, high-throughput phenotyping
to generate and test mechanistic models to explain the genetic diversity
of photosynthetic responses to abiotic stress. We assessed a population
of recombinant inbred lines (RILs) of cowpea (Vigna unguiculata. (L.)
Walp.) with significant differences in a range of photosynthetic
responses to chilling. We found well-defined, colocalized (overlapping)
QTL intervals for photosynthetic parameters, suggesting linkages among
the redox states of Q\textsubscript{A}, the thylakoid \emph{pmf},
through effects on cyclic electron flow and photodamage to PSII. We
propose that these genetic variations optimize photosynthesis in the
tolerant lines under low temperatures, preventing recombination
reactions within Photosystem II that can lead to deleterious
\textsuperscript{1}O\textsubscript{2} production. By contrast, we did
not observe linkages to PSI redox state, PSI photodamage or ATP synthase
activity, or nyctinastic (diurnally controlled) leaf movements, likely
indicating that several proposed models likely do not contribute to the
genetic control of photosynthesis at low temperature in our mapping
panel. The identified QTL intervals include a range of potential
causative genetic components, with direct applications to breeding of
photosynthesis for more climate-resilient productivity.%
\end{abstract}%



\sloppy




\selectlanguage{english}
\FloatBarrier
\end{document}

