Genetic structure
The STRUCTURE analyses carried out with the 3RAD data set displayed the
highest value of Ln P(D) at K = 7, though the Evanno method had its
highest value at K = 3 (Figure 3). At K= 2, we recovered one cluster
with members of E. ruidum sp. 1 and E. ruidum sp. 2 and
another with those of E. ruidum spp. 3, 4 and 2x3. At K= 3 the
specimens of E. ruidum sp. 1 from Guatemala and Trinidad and
Tobago formed a relatively well-differentiated cluster. At subsequent K
values, the specimens from Pinotepa and localities in Guerrero in
Mexico, and the ones from Trinidad and Tobago were each recovered as
exclusive clusters, whereas at K= 7 and 8 the specimens representingE. ruidum sp. 3 (except the one from Guerrero), E. ruidumsp. 4, E. ruidum sp. 2 from Quintana Roo, Mexico and Colombia,
and the one from Guatemala each formed well-differentiated clusters.