Species delineation
The BPP analyses conducted using the 3RAD data set significantly
supported the species model that considered five evolutionary lineages
(PP > 0.95; Table S3), the four putative species proposed
by Aguilar Velasco et al. (2016), E. ruidum spp. 1-4, and the
specimens from Pinotepa and the state of Guerrero that these authors
regarded as E. ruidum sp. 2x3 and as part of E. ruidum sp.
3, respectively. In contrast, when we assigned the samples according to
their geographic proximity, our results widely varied (Table S4).
The Bayes factor comparisons of the two tested species delimitation
models using the phased UCE data consistently favored a five species
model, with E. ruidum spp. 1, 2, 3 and 4, as well as the
specimens from Pinotepa and Guerrero, Mexico, each representing separate
species (Table S4).