Determination of MDA, H2O2 and OFR
content
To determine malonaldehyde (MDA) content in soybean leaves, we referred
to the protocol of Hasanuzzaman et al. (2017) with slight modification.
The absorbance at 532 and 600 nm were measured on a spectrophotometer.
Hydrogen peroxide (H2O2) content was
measured according to the method of Hasanuzzaman et al. (2017). The
absorbance of the obtained solution was read at 415 nm. The production
of an oxygen free radical (OFR) was determined using an OFR reagent kit
(Comin Biotechnology, Suzhou, China). OFR scavenging ability was
calculated using OD530.