Changes of endogenous H2S, lipid membrane peroxidation, hydrogen peroxide, and superoxide anion in leaves of soybean plants under water deficiency
The endogenous H2S contents were reduced by NaHS, Q8 and Q8+NaHS treatments condition under water deficiency (Fig. 5A). Under NW and SW conditions, the endogenous H2S content of Q8+NaHS treatment was higher than that of control, and the endogenous H2S content was not markedly regulated by Q8 and Q8+NaHS treatments under MW condition (Fig. 5A). With the decrease of water content, the MDA content and OFR content were obviously increased by four treatments (Fig. 5B and 5D). Under SW condition, membrane lipid peroxidation was increased by 20.5% and 14.7% in Q8 and Q8+NaHS treatments, respectively, compared with control (Fig. 5B). The OFR content was reduced by Q8+NaHS treatment compared with the control under SW condition (Fig. 5D). With the decreases of water content, the H2O2 content was not obviously influenced by control treatment. However, the H2O2 content of NaHS, Q8 and Q8+NaHS treatments displayed a tendency to increase initially and then reduce with the decrease of water content. In addition, under three water conditions, the H2O2 content of the Q8+NaHS treatment was considerably lower than the control (Fig. 5C).