Determination of antioxidant
The activities of superoxide dismutase (SOD), peroxidase (POD), catalase
(CAT), and ascorbate peroxidase (APX) were measured using enzymatic
activity assay kits (Comin Biotechnology, Suzhou, China). The whole
procedure was conducted following the instructions of the manufacturer.
The SOD, POD, CAT, and APX activities were measured with an EPOCH
ultraviolet spectrophotometer (BioTek, Vermont, U.S.A) at
OD560, OD470, OD240 and
OD290, respectively.