2.7.1. Sample preparation
Aqueous extracts of Senjed peel were added to the 100 mL cold-pressed
sesame oil at concentrations of 0 (T0) (as negative control without
antioxidant), 100 (T100), 200 (T200), 500 (T500) and 750 ppm (T750)
(respectively, 0%, 0.01%, 0.02%, 0.05%, and 0.075% of fat content).
Synthetic antioxidant TBHQ was employed at their legal limit of 200 ppm
(0.02% of fat content) (as positive control) in cold-pressed sesame
oil. All of prepared oil samples were filled in PET containers and
stored at 25 ℃ ± 5 and 30–35% relative humidity.