2.5. Evaluation of antioxidant activity of Senjed peel aqueous extracts
The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay was performed according to Hussain et al. (2018). The obtained extracts and the synthetic antioxidant TBHQ (0.1 mL of each) was allowed to react with 3.9 mL of DPPH solution (6 × 10-5 M) and absorbance was measured at 515 nm after 30 min. The DPPH scavenging percentage was calculated according to the following formula.
\(\text{DPPHs\ Scavenging\ capacity\ }\left(\%\right)=\ \frac{A_{\text{control}}-\ A_{\text{sample}}}{A_{\text{control}}}\ \times 100\)(1)
A = absorbance at 515 nm.