2.2. Preparation of Senjed peel aqueous extracts
Using Soxhlet extractor, 20 g of dried and ground Senjed peel was mixed with 200 ml of double distilled water in a round bottom flask and refluxed for about 8 h at 100 °C. Obtained liquid extracts were separated from the solid residue by vacuum filtration, vacuum-evaporated (Heidolph Rotavapor, Germany, 40 ℃, vacuum 0.6 bar and rpm 50) to obtain the concentrated extract. Semi-dried extracts placed in amber-colored glasses, flushed with nitrogen, and stored at 4 ℃ until the analyses.