Phylogenetic analysis
Phylogenetic evaluation of HA and NA genes was done using Mega 7,
neighbour-joining method, phylogeny test by bootstrap method and 1000
replications, Kimura 2 substitution model and partial deletion (site
coverage cutoff 5%). The viruses were compared with A(H1N1)pdm09
reference viruses according to McCauley et al. (2019) (11). The
identified substitutions (see FluSurver analysis) were assigned to
protein domains (region of antigenic sites and receptor binding sites)
according to the FluSurver database and Igarashi et al. and Yang et al.
(5, 12).