2, 7-dichlorodihydrofluorescein diacetate (H2DCFDA) staining
Reactive oxygen species (ROS) in Th17 cells were assessed by H2DCFDA staining. Th17 cells were incubated in pre-warmed PBS containing 10 μM H2DCFDA (ThermoFisher) for 15 minutes at 37°C. After two washes with PBS, cells were suspended in PBS and the green fluorescence was measured on the flow cytometer.